Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABJR13_RS05340 Genome accession   NZ_CP169360
Coordinates   1125552..1126031 (-) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain Mh184.20180323.PV.C     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1120552..1131031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABJR13_RS05315 (ABJR13_001064) yacG 1121454..1121639 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  ABJR13_RS05320 (ABJR13_001065) coaE 1121621..1122256 (-) 636 WP_020831305.1 dephospho-CoA kinase -
  ABJR13_RS05325 (ABJR13_001066) - 1122292..1122948 (-) 657 WP_006248404.1 prepilin peptidase -
  ABJR13_RS05330 (ABJR13_001067) pilC 1122951..1124147 (-) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  ABJR13_RS05335 (ABJR13_001068) pilB 1124131..1125531 (-) 1401 WP_040080755.1 GspE/PulE family protein Machinery gene
  ABJR13_RS05340 (ABJR13_001069) pilA 1125552..1126031 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  ABJR13_RS05345 (ABJR13_001070) radA 1126212..1127591 (+) 1380 WP_040080753.1 DNA repair protein RadA Machinery gene
  ABJR13_RS05350 (ABJR13_001071) - 1127646..1127876 (-) 231 WP_006248398.1 YgjV family protein -
  ABJR13_RS05355 (ABJR13_001072) folA 1127996..1128490 (-) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  ABJR13_RS05360 (ABJR13_001073) proB 1128602..1129693 (+) 1092 WP_006251346.1 glutamate 5-kinase -
  ABJR13_RS05365 (ABJR13_001074) - 1129693..1130457 (+) 765 WP_006248395.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=1050948 ABJR13_RS05340 WP_006248401.1 1125552..1126031(-) (pilA) [Mannheimia haemolytica strain Mh184.20180323.PV.C]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=1050948 ABJR13_RS05340 WP_006248401.1 1125552..1126031(-) (pilA) [Mannheimia haemolytica strain Mh184.20180323.PV.C]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491

Multiple sequence alignment