Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABGU74_RS01600 Genome accession   NZ_OZ040756
Coordinates   346141..347637 (+) Length   498 a.a.
NCBI ID   WP_001040681.1    Uniprot ID   -
Organism   Escherichia coli isolate 30859_5#287     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 341141..352637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABGU74_RS01570 tusA 341388..341633 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  ABGU74_RS01575 zntA 341735..343933 (-) 2199 WP_000106576.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  ABGU74_RS01580 yhhN 344007..344633 (-) 627 WP_000964718.1 lysoplasmalogenase -
  ABGU74_RS01585 yhhM 344774..345133 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  ABGU74_RS01590 yhhL 345136..345405 (-) 270 WP_001295207.1 DUF1145 family protein -
  ABGU74_RS01595 rsmD 345395..345991 (-) 597 WP_000743189.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  ABGU74_RS01600 pilA 346141..347637 (+) 1497 WP_001040681.1 signal recognition particle-docking protein FtsY Machinery gene
  ABGU74_RS01605 ftsE 347640..348308 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  ABGU74_RS01610 ftsX 348301..349359 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  ABGU74_RS01615 rpoH 349604..350458 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  ABGU74_RS01620 livJ 350729..351832 (+) 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  ABGU74_RS01625 ataR 351981..352247 (+) 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54672.49 Da        Isoelectric Point: 4.1449

>NTDB_id=1050862 ABGU74_RS01600 WP_001040681.1 346141..347637(+) (pilA) [Escherichia coli isolate 30859_5#287]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQEQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGLRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=1050862 ABGU74_RS01600 WP_001040681.1 346141..347637(+) (pilA) [Escherichia coli isolate 30859_5#287]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGAGCAAGAGCCTGTGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGATCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACAGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCTGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment