Detailed information NTG1050839

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: ABJR16_RS04800
• Genome accession: NZ_CP169355
• Coordinates: 1017014..1017493 (-)
• Length: 159 a.a.
• NCBI ID: WP_006248401.1
• Uniprot ID: A0A249A0S4
• Organism: Mannheimia haemolytica strain Mh184.20180323.PV.A
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1012014..1022493

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABJR16_RS04775 (ABJR16_000956)	yacG	1012916..1013101 (-)	186	WP_006248406.1	DNA gyrase inhibitor YacG	-
ABJR16_RS04780 (ABJR16_000957)	coaE	1013083..1013718 (-)	636	WP_020831305.1	dephospho-CoA kinase	-
ABJR16_RS04785 (ABJR16_000958)	-	1013754..1014410 (-)	657	WP_006248404.1	prepilin peptidase	-
ABJR16_RS04790 (ABJR16_000959)	pilC	1014413..1015609 (-)	1197	WP_006248403.1	type II secretion system F family protein	Machinery gene
ABJR16_RS04795 (ABJR16_000960)	pilB	1015593..1016993 (-)	1401	WP_040080755.1	GspE/PulE family protein	Machinery gene
ABJR16_RS04800 (ABJR16_000961)	pilA	1017014..1017493 (-)	480	WP_006248401.1	prepilin peptidase-dependent pilin	Machinery gene
ABJR16_RS04805 (ABJR16_000962)	radA	1017674..1019052 (+)	1379	Protein_930	DNA repair protein RadA	-
ABJR16_RS04810 (ABJR16_000963)	-	1019107..1019337 (-)	231	WP_006248398.1	YgjV family protein	-
ABJR16_RS04815 (ABJR16_000964)	folA	1019457..1019951 (-)	495	WP_006248397.1	type 3 dihydrofolate reductase	-
ABJR16_RS04820 (ABJR16_000965)	proB	1020063..1021154 (+)	1092	WP_006251346.1	glutamate 5-kinase	-
ABJR16_RS04825 (ABJR16_000966)	-	1021154..1021918 (+)	765	WP_006248395.1	TSUP family transporter	-

Sequence

Protein

• Length: 159 a.a.
• Molecular weight: 16841.15 Da
• Isoelectric Point: 8.7964

>NTDB_id=1050839 ABJR16_RS04800 WP_006248401.1 1017014..1017493(-) (pilA) [Mannheimia haemolytica strain Mh184.20180323.PV.A]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide

• Length: 480 bp

>NTDB_id=1050839 ABJR16_RS04800 WP_006248401.1 1017014..1017493(-) (pilA) [Mannheimia haemolytica strain Mh184.20180323.PV.A]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (48-148)

• Prokaryotic N-terminal methylation motif (N_methyl) (16-38)

3D structure

• AlphaFold DB: A0A249A0S4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Glaesserella parasuis strain SC1401	66.038	100	0.66
pilA	Haemophilus influenzae 86-028NP	56.429	88.05	0.497
pilA	Haemophilus influenzae Rd KW20	54.93	89.308	0.491