Detailed information NTG1044641

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACEPWM_RS07180
• Genome accession: NZ_CP168642
• Coordinates: 1528493..1528990 (-)
• Length: 165 a.a.
• NCBI ID: WP_001838632.1
• Uniprot ID: A0A6A9GZK7
• Organism: Staphylococcus aureus strain NCCP11854
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1523493..1533990

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEPWM_RS07155 (ACEPWM_07155)	gcvPB	1523655..1525127 (-)	1473	WP_000202177.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
ACEPWM_RS07160 (ACEPWM_07160)	gcvPA	1525120..1526466 (-)	1347	WP_000019687.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACEPWM_RS07165 (ACEPWM_07165)	gcvT	1526486..1527577 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACEPWM_RS07170 (ACEPWM_07170)	-	1527736..1528260 (-)	525	WP_001015116.1	shikimate kinase	-
ACEPWM_RS07175 (ACEPWM_07175)	-	1528250..1528396 (-)	147	WP_001789879.1	hypothetical protein	-
ACEPWM_RS07180 (ACEPWM_07180)	comGF	1528493..1528990 (-)	498	WP_001838632.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACEPWM_RS07185 (ACEPWM_07185)	comGE	1528908..1529207 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACEPWM_RS07190 (ACEPWM_07190)	comGD	1529194..1529640 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACEPWM_RS07195 (ACEPWM_07195)	comGC	1529618..1529929 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
ACEPWM_RS07200 (ACEPWM_07200)	comGB	1529943..1531013 (-)	1071	WP_000776407.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACEPWM_RS07205 (ACEPWM_07205)	comGA	1530985..1531959 (-)	975	WP_000697218.1	competence type IV pilus ATPase ComGA	Machinery gene
ACEPWM_RS07210 (ACEPWM_07210)	-	1532011..1532634 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACEPWM_RS07215 (ACEPWM_07215)	-	1532631..1532960 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACEPWM_RS07220 (ACEPWM_07220)	-	1532960..1533946 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19184.87 Da
• Isoelectric Point: 10.2192

>NTDB_id=1044641 ACEPWM_RS07180 WP_001838632.1 1528493..1528990(-) (comGF) [Staphylococcus aureus strain NCCP11854]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNNIENREQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1044641 ACEPWM_RS07180 WP_001838632.1 1528493..1528990(-) (comGF) [Staphylococcus aureus strain NCCP11854]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCTCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACAATATTGAAAATAGGGAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (68-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

3D structure

• AlphaFold DB: A0A6A9GZK7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	96.364	100	0.964
comGF	Staphylococcus aureus MW2	96.364	100	0.964