Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   ACD268_RS10075 Genome accession   NZ_CP168299
Coordinates   1959376..1959837 (-) Length   153 a.a.
NCBI ID   WP_000250534.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain FC1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1954376..1964837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACD268_RS10040 (ACD268_10040) - 1954680..1955504 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  ACD268_RS10045 (ACD268_10045) rnpA 1955479..1955850 (-) 372 WP_000739246.1 ribonuclease P protein component -
  ACD268_RS10050 (ACD268_10050) - 1955867..1955998 (-) 132 WP_000768904.1 hypothetical protein -
  ACD268_RS10055 (ACD268_10055) - 1955999..1957189 (-) 1191 WP_330789279.1 acetate kinase -
  ACD268_RS10060 (ACD268_10060) comYH 1957240..1958193 (-) 954 WP_330789278.1 class I SAM-dependent methyltransferase Machinery gene
  ACD268_RS10065 (ACD268_10065) - 1958254..1958848 (-) 595 Protein_1958 class I SAM-dependent methyltransferase -
  ACD268_RS10070 (ACD268_10070) comGG/cglG 1958985..1959398 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACD268_RS10075 (ACD268_10075) comGF/cglF 1959376..1959837 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACD268_RS10080 (ACD268_10080) comGE/cglE 1959800..1960102 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACD268_RS10085 (ACD268_10085) comGD/cglD 1960065..1960469 (-) 405 WP_000588024.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACD268_RS10090 (ACD268_10090) comGC/cglC 1960462..1960728 (-) 267 WP_050129046.1 competence type IV pilus major pilin ComGC Machinery gene
  ACD268_RS10095 (ACD268_10095) - 1960730..1960987 (-) 258 WP_000698512.1 hypothetical protein -
  ACD268_RS10100 (ACD268_10100) lytA 1961219..1962175 (-) 957 WP_373381835.1 N-acetylmuramoyl-L-alanine amidase LytA -
  ACD268_RS10105 (ACD268_10105) - 1962179..1962511 (-) 333 WP_001186206.1 phage holin -
  ACD268_RS10110 (ACD268_10110) - 1962515..1962931 (-) 417 WP_001165344.1 phage holin family protein -
  ACD268_RS10115 (ACD268_10115) - 1962941..1963291 (-) 351 WP_000852249.1 hypothetical protein -
  ACD268_RS10120 (ACD268_10120) - 1963294..1963497 (-) 204 WP_001091107.1 hypothetical protein -
  ACD268_RS10125 (ACD268_10125) - 1963478..1963594 (-) 117 Protein_1970 dihydrodipicolinate reductase -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17972.67 Da        Isoelectric Point: 9.6092

>NTDB_id=1043746 ACD268_RS10075 WP_000250534.1 1959376..1959837(-) (comGF/cglF) [Streptococcus pneumoniae strain FC1]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1043746 ACD268_RS10075 WP_000250534.1 1959376..1959837(-) (comGF/cglF) [Streptococcus pneumoniae strain FC1]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGATGATTTCCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGACTCAAATCTGTACGGATTACAGAGGACAATCAACTGGTTCGCTTTCATT
TCCAGTTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

100

100

1
  comGF/cglF Streptococcus pneumoniae D39

100

100

1
  comGF/cglF Streptococcus pneumoniae R6

100

100

1
  comGF/cglF Streptococcus pneumoniae TIGR4

100

100

1
  comGF/cglF Streptococcus mitis SK321

90.85

100

0.909
  comGF/cglF Streptococcus mitis NCTC 12261

89.542

100

0.895

Multiple sequence alignment