Detailed information NTG1042048

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACCK16_RS07510
• Genome accession: NZ_CP168089
• Coordinates: 1572768..1573265 (-)
• Length: 165 a.a.
• NCBI ID: WP_001796472.1
• Uniprot ID: A0A5F0HJ55
• Organism: Staphylococcus aureus strain CUBIST-23
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1567768..1578265

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACCK16_RS07485 (ACCK16_07485)	gcvPB	1567930..1569402 (-)	1473	WP_000202176.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
ACCK16_RS07490 (ACCK16_07490)	gcvPA	1569395..1570741 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACCK16_RS07495 (ACCK16_07495)	gcvT	1570761..1571852 (-)	1092	WP_000093344.1	glycine cleavage system aminomethyltransferase GcvT	-
ACCK16_RS07500 (ACCK16_07500)	-	1572011..1572535 (-)	525	WP_001015120.1	shikimate kinase	-
ACCK16_RS07505 (ACCK16_07505)	-	1572525..1572671 (-)	147	WP_001789879.1	hypothetical protein	-
ACCK16_RS07510 (ACCK16_07510)	comGF	1572768..1573265 (-)	498	WP_001796472.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACCK16_RS07515 (ACCK16_07515)	comGE	1573183..1573482 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACCK16_RS07520 (ACCK16_07520)	comGD	1573469..1573915 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACCK16_RS07525 (ACCK16_07525)	comGC	1573893..1574204 (-)	312	WP_000472255.1	competence type IV pilus major pilin ComGC	Machinery gene
ACCK16_RS07530 (ACCK16_07530)	comGB	1574218..1575289 (-)	1072	Protein_1477	competence type IV pilus assembly protein ComGB	-
ACCK16_RS07535 (ACCK16_07535)	comGA	1575261..1576235 (-)	975	WP_302287794.1	competence type IV pilus ATPase ComGA	Machinery gene
ACCK16_RS07540 (ACCK16_07540)	-	1576287..1576910 (-)	624	WP_001223009.1	MBL fold metallo-hydrolase	-
ACCK16_RS07545 (ACCK16_07545)	-	1576907..1577236 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACCK16_RS07550 (ACCK16_07550)	-	1577236..1578222 (-)	987	WP_000161307.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19169.89 Da
• Isoelectric Point: 10.1358

>NTDB_id=1042048 ACCK16_RS07510 WP_001796472.1 1572768..1573265(-) (comGF) [Staphylococcus aureus strain CUBIST-23]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1042048 ACCK16_RS07510 WP_001796472.1 1572768..1573265(-) (comGF) [Staphylococcus aureus strain CUBIST-23]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (68-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

3D structure

• AlphaFold DB: A0A5F0HJ55

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	95.758	100	0.958