Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   AB8613_RS10065 Genome accession   NZ_CP167894
Coordinates   2219803..2220417 (+) Length   204 a.a.
NCBI ID   WP_372383793.1    Uniprot ID   -
Organism   Vibrio sp. BS-M-Sm-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2214803..2225417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8613_RS10045 (AB8613_10045) - 2214898..2215077 (-) 180 WP_017096544.1 hypothetical protein -
  AB8613_RS10050 (AB8613_10050) oxyR 2215076..2215969 (+) 894 WP_372057601.1 DNA-binding transcriptional regulator OxyR -
  AB8613_RS10055 (AB8613_10055) - 2216081..2218642 (-) 2562 WP_372383792.1 penicillin-binding protein 1A -
  AB8613_RS10060 (AB8613_10060) pilM 2218794..2219819 (+) 1026 WP_285953307.1 type IV pilus assembly protein PilM Machinery gene
  AB8613_RS10065 (AB8613_10065) pilN 2219803..2220417 (+) 615 WP_372383793.1 PilN domain-containing protein Machinery gene
  AB8613_RS10070 (AB8613_10070) pilO 2220410..2221039 (+) 630 WP_146492434.1 type 4a pilus biogenesis protein PilO Machinery gene
  AB8613_RS10075 (AB8613_10075) pilP 2221026..2221544 (+) 519 WP_210446842.1 pilus assembly protein PilP Machinery gene
  AB8613_RS10080 (AB8613_10080) pilQ 2221580..2223298 (+) 1719 WP_372383794.1 type IV pilus secretin PilQ Machinery gene
  AB8613_RS10085 (AB8613_10085) aroK 2223475..2223993 (+) 519 WP_017061125.1 shikimate kinase AroK -
  AB8613_RS10090 (AB8613_10090) aroB 2224019..2225107 (+) 1089 WP_017061124.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 23351.74 Da        Isoelectric Point: 9.3212

>NTDB_id=1040119 AB8613_RS10065 WP_372383793.1 2219803..2220417(+) (pilN) [Vibrio sp. BS-M-Sm-2]
MLHQINLLPWRDEIRAQHKKRFVHLVILGVIIALGGQWAVGNYFHDQQAKQQARLNYLNQYIAELDRQIQSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKGVEMHSIV
HNRKRFNKEFQTFKVSFVFSPVSLENTTAESTAASRKEKGAHHG

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1040119 AB8613_RS10065 WP_372383793.1 2219803..2220417(+) (pilN) [Vibrio sp. BS-M-Sm-2]
ATGTTGCATCAAATTAACCTGCTGCCTTGGCGTGATGAGATTCGTGCTCAGCATAAGAAGCGTTTTGTTCATCTGGTCAT
TCTCGGTGTCATCATTGCGCTTGGTGGGCAATGGGCGGTCGGTAACTATTTTCACGACCAACAAGCCAAGCAGCAAGCGC
GCCTTAACTATCTCAATCAATACATCGCTGAGCTCGACCGACAGATTCAGTCATTAAAAGTCGCCGAACAAGAGCACAAA
GCCATTCTCACTCGACTAGATGTGGTGGAGTCTTTACAGCTTGGCCGCAATAAGACCACCGACTTCATGAACCTAATGCC
GGAGCTGATTCCTGAGGGTGTATATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGTATCAGCGACA
GTACGGCTCGTCTCGCCACGATGTTGGATAACCTCGAGCGTTCAGATTCTCTGAAAGGGGTTGAGATGCACTCTATCGTT
CATAACCGCAAACGCTTCAATAAAGAGTTTCAGACCTTCAAGGTCTCTTTTGTGTTCAGCCCTGTGTCTTTAGAAAACAC
AACCGCAGAGAGCACTGCCGCGAGCAGAAAAGAGAAGGGGGCACATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

60.976

100

0.613
  pilN Vibrio campbellii strain DS40M4

63.441

91.176

0.578

Multiple sequence alignment