Detailed information NTG1040118

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AB8613_RS10060
• Genome accession: NZ_CP167894
• Coordinates: 2218794..2219819 (+)
• Length: 341 a.a.
• NCBI ID: WP_285953307.1
• Uniprot ID: -
• Organism: Vibrio sp. BS-M-Sm-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2213794..2224819

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB8613_RS10040 (AB8613_10040)	-	2214125..2214853 (-)	729	WP_017061133.1	glutathione peroxidase	-
AB8613_RS10045 (AB8613_10045)	-	2214898..2215077 (-)	180	WP_017096544.1	hypothetical protein	-
AB8613_RS10050 (AB8613_10050)	oxyR	2215076..2215969 (+)	894	WP_372057601.1	DNA-binding transcriptional regulator OxyR	-
AB8613_RS10055 (AB8613_10055)	-	2216081..2218642 (-)	2562	WP_372383792.1	penicillin-binding protein 1A	-
AB8613_RS10060 (AB8613_10060)	pilM	2218794..2219819 (+)	1026	WP_285953307.1	type IV pilus assembly protein PilM	Machinery gene
AB8613_RS10065 (AB8613_10065)	pilN	2219803..2220417 (+)	615	WP_372383793.1	PilN domain-containing protein	Machinery gene
AB8613_RS10070 (AB8613_10070)	pilO	2220410..2221039 (+)	630	WP_146492434.1	type 4a pilus biogenesis protein PilO	Machinery gene
AB8613_RS10075 (AB8613_10075)	pilP	2221026..2221544 (+)	519	WP_210446842.1	pilus assembly protein PilP	Machinery gene
AB8613_RS10080 (AB8613_10080)	pilQ	2221580..2223298 (+)	1719	WP_372383794.1	type IV pilus secretin PilQ	Machinery gene
AB8613_RS10085 (AB8613_10085)	aroK	2223475..2223993 (+)	519	WP_017061125.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38210.66 Da
• Isoelectric Point: 6.7735

>NTDB_id=1040118 AB8613_RS10060 WP_285953307.1 2218794..2219819(+) (pilM) [Vibrio sp. BS-M-Sm-2]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRAYALTKAGFKP
VVVDTQAHGLLNIWQLASRLYPEKKNWLLVDVGIDQTSLGVIPQNSVPFYKDIAFGTQDLRSSDNPSDTEGVLGTAEDTH
QFIVNLIEKLKRQLQLYSSGNAQQPISGIWLMGEGASIPMVTEELERHFQLSCESLNPLSLFENKVAKRRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=1040118 AB8613_RS10060 WP_285953307.1 2218794..2219819(+) (pilM) [Vibrio sp. BS-M-Sm-2]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGACTGCCTTTTGGCTGTCGCAACGTCGCCATTTCGGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCTATCTATCAAACCTTCGCCCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTATCAGGTATACGCCACTCGTAAGGAAGTGGTGGATAGCCGAGCTTATGCATTAACTAAGGCTGGTTTTAAACCT
GTGGTGGTAGATACACAGGCACATGGGCTGCTCAATATCTGGCAACTAGCCTCGCGTCTGTATCCCGAAAAGAAAAACTG
GCTGCTGGTGGATGTTGGCATCGACCAAACGTCGCTAGGAGTTATCCCGCAGAATTCAGTGCCTTTCTATAAAGACATCG
CTTTTGGTACTCAAGATCTTCGCAGTTCGGATAACCCAAGTGATACAGAAGGTGTGCTCGGCACGGCTGAAGATACCCAT
CAATTCATTGTTAATTTGATTGAGAAGCTCAAGCGTCAATTACAGCTTTATTCATCGGGAAATGCGCAGCAACCTATCTC
TGGGATCTGGCTGATGGGTGAGGGTGCCAGCATTCCTATGGTAACCGAAGAGCTAGAGCGCCATTTTCAGCTGAGCTGTG
AGTCATTGAATCCTTTGTCTCTGTTTGAGAACAAGGTCGCCAAGAGACGTCGACTGCCAATGGACTGGCAACACTTTGGC
ATTGCCGCGGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-220)

• Type IV pilus assembly protein PilM; (PilM_2) (242-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	54.277	99.413	0.54
pilM	Vibrio campbellii strain DS40M4	48.538	100	0.487