Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   KJP64_RS06985 Genome accession   NZ_OX419565
Coordinates   1329236..1330585 (-) Length   449 a.a.
NCBI ID   WP_009967069.1    Uniprot ID   O34358
Organism   Bacillus subtilis isolate NRS6145     
Function   degrading CSP; selective degradation of ComEA and ComEC (predicted from homology)   
Competence regulation

Genomic Context

Location: 1324236..1335585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP64_RS06960 (NRS6145_06870) mhqA 1324418..1325368 (+) 951 WP_003245086.1 ring-cleaving dioxygenase -
  KJP64_RS06965 - 1325477..1325572 (+) 96 Protein_1310 hypothetical protein -
  KJP64_RS06970 (NRS6145_06875) ykcB 1325585..1327735 (+) 2151 WP_167560991.1 glycosyltransferase family 39 protein -
  KJP64_RS06975 (NRS6145_06880) ykcC 1327747..1328718 (+) 972 WP_015715747.1 glycosyltransferase family 2 protein -
  KJP64_RS06980 (NRS6145_06885) - 1328902..1329078 (-) 177 WP_003245413.1 hypothetical protein -
  KJP64_RS06985 (NRS6145_06890) htrA 1329236..1330585 (-) 1350 WP_009967069.1 serine protease HtrA Regulator
  KJP64_RS06990 (NRS6145_06895) proG 1330754..1331572 (+) 819 WP_032721395.1 pyrroline-5-carboxylate reductase ProG -
  KJP64_RS06995 (NRS6145_06900) dppA 1331701..1332525 (+) 825 WP_033884945.1 D-aminopeptidase DppA -
  KJP64_RS07000 (NRS6145_06905) dppB 1332542..1333468 (+) 927 WP_003245446.1 dipeptide ABC transporter permease DppB -
  KJP64_RS07005 (NRS6145_06910) dppC 1333474..1334436 (+) 963 WP_032721397.1 dipeptide ABC transporter permease DppC -
  KJP64_RS07010 (NRS6145_06915) dppD 1334441..1335448 (+) 1008 WP_032721399.1 dipeptide ABC transporter ATP-binding subunit DppD -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 47714.85 Da        Isoelectric Point: 4.5990

>NTDB_id=1039964 KJP64_RS06985 WP_009967069.1 1329236..1330585(-) (htrA) [Bacillus subtilis isolate NRS6145]
MDNYRDENRTKGNENEVFLTKENDQSASYSARNVIHDQEKKKRGFGWFRPLLGGVIGGSLALGIYTFTPLGDHDSQDTAK
QSSSQQQTQSVTATSTSSESKKSSSSSSAFKSEDSSKISDMVEDLSPAIVGITNLQAQSNSSLFGSSSSDSSEDTESGSG
SGVIFKKENGKAYIITNNHVVEGASSLKVSLYDGTEVTAKLVGSDSLTDLAVLQISDDHVTKVANFGDSSDLRTGETVIA
IGDPLGKDLSRTVTQGIVSGVDRTVSMSTSAGETSINVIQTDAAINPGNSGGPLLNTDGKIVGINSMKISEDDVEGIGFA
IPSNDVKPIAEELLSKGQIERPYIGVSMLDLEQVPQNYQEGTLGLFGSQLNKGVYIREVASGSPAEKAGLKAEDIIIGLK
GKEIDTGSELRNILYKDAKIGDTVEVKILRNGKEMTKKIKLDQKEEKTS

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1039964 KJP64_RS06985 WP_009967069.1 1329236..1330585(-) (htrA) [Bacillus subtilis isolate NRS6145]
ATGGATAACTATCGTGATGAAAACAGAACGAAAGGTAATGAGAATGAGGTCTTTTTAACGAAAGAGAACGATCAGAGCGC
CTCCTACTCGGCCCGCAATGTCATTCATGATCAGGAGAAGAAAAAACGAGGATTCGGATGGTTCAGACCGTTGCTTGGCG
GAGTGATCGGCGGCAGTCTTGCTCTTGGCATTTACACGTTTACACCGCTTGGTGACCATGATTCTCAGGACACTGCAAAA
CAATCATCCAGCCAGCAGCAAACGCAATCTGTTACAGCAACAAGCACCTCCTCTGAATCTAAAAAAAGCTCAAGCAGCTC
ATCTGCATTCAAGAGCGAGGACTCTTCTAAAATCTCAGATATGGTAGAAGACCTTTCACCAGCGATTGTCGGTATTACAA
ATCTTCAGGCACAATCAAACAGCTCTTTGTTCGGCTCTAGTTCTTCTGATTCCAGCGAAGATACAGAAAGCGGTTCAGGG
TCAGGTGTCATTTTCAAAAAAGAGAATGGCAAGGCTTATATCATTACAAATAACCACGTCGTAGAAGGGGCATCATCACT
GAAGGTATCTTTATATGACGGCACTGAGGTTACTGCAAAGCTGGTAGGCAGTGACTCGTTAACTGATTTAGCCGTCCTCC
AAATCAGTGATGACCACGTCACAAAAGTGGCAAACTTCGGTGATTCATCTGATCTTAGAACAGGCGAGACCGTTATTGCG
ATTGGGGATCCGCTTGGAAAAGACCTGTCCCGCACAGTAACACAAGGAATTGTAAGCGGCGTGGACAGAACGGTTTCAAT
GTCTACATCAGCCGGCGAAACGAGCATTAACGTCATTCAGACAGACGCAGCAATTAATCCAGGTAACAGCGGCGGTCCTT
TGTTAAATACAGACGGCAAAATTGTCGGCATTAACAGTATGAAAATCAGTGAGGATGATGTTGAGGGTATCGGATTCGCC
ATTCCAAGCAATGACGTAAAACCGATTGCTGAAGAATTGCTGTCTAAAGGACAAATTGAACGTCCATATATCGGTGTCAG
CATGCTTGATCTAGAGCAAGTGCCGCAAAATTACCAAGAAGGCACACTCGGCCTGTTCGGCAGCCAGCTGAATAAAGGCG
TTTACATCCGTGAGGTCGCTTCAGGCTCTCCTGCTGAAAAGGCCGGATTAAAAGCGGAGGATATTATCATCGGCCTAAAA
GGTAAAGAAATTGATACAGGCAGTGAATTGCGCAATATCTTATATAAAGACGCAAAGATCGGTGATACCGTTGAAGTGAA
AATTCTCCGAAACGGCAAAGAAATGACGAAAAAAATTAAACTGGATCAAAAAGAAGAGAAAACTTCGTAA

Domains

Predicted by InterProScan.

PDZ domain (PDZ_2)

(342-441)

Trypsin-like peptidase domain (Trypsin_2)

(160-304)

Peptidase family M50 (Peptidase_M50)

(366-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34358

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus pneumoniae Rx1

41.045

89.532

0.367
  htrA Streptococcus pneumoniae D39

41.045

89.532

0.367
  htrA Streptococcus pneumoniae R6

41.045

89.532

0.367
  htrA Streptococcus pneumoniae TIGR4

41.045

89.532

0.367
  htrA Streptococcus mitis NCTC 12261

39.563

91.759

0.363