Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   QUF32_RS14350 Genome accession   NZ_AP028128
Coordinates   3043742..3044332 (-) Length   196 a.a.
NCBI ID   WP_020329444.1    Uniprot ID   -
Organism   Vibrio fluvialis strain IDH05335     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3038742..3049332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF32_RS14325 (KKIDH5335_27500) aroB 3039077..3040162 (-) 1086 WP_024374091.1 3-dehydroquinate synthase -
  QUF32_RS14330 (KKIDH5335_27510) aroK 3040190..3040708 (-) 519 WP_004728569.1 shikimate kinase AroK -
  QUF32_RS14335 (KKIDH5335_27520) pilQ 3040896..3042632 (-) 1737 WP_286236258.1 type IV pilus secretin PilQ family protein Machinery gene
  QUF32_RS14340 (KKIDH5335_27530) pilP 3042657..3043169 (-) 513 WP_020329446.1 pilus assembly protein PilP Machinery gene
  QUF32_RS14345 (KKIDH5335_27540) pilO 3043159..3043749 (-) 591 WP_020329445.1 type 4a pilus biogenesis protein PilO Machinery gene
  QUF32_RS14350 (KKIDH5335_27550) pilN 3043742..3044332 (-) 591 WP_020329444.1 PilN domain-containing protein Machinery gene
  QUF32_RS14355 (KKIDH5335_27560) pilM 3044322..3045326 (-) 1005 WP_020329442.1 type IV pilus assembly protein PilM Machinery gene
  QUF32_RS14360 (KKIDH5335_27570) - 3045469..3047949 (+) 2481 WP_222733502.1 PBP1A family penicillin-binding protein -
  QUF32_RS14365 (KKIDH5335_27580) oxyR 3048010..3048906 (-) 897 WP_020329438.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22305.74 Da        Isoelectric Point: 9.8751

>NTDB_id=103957 QUF32_RS14350 WP_020329444.1 3043742..3044332(-) (pilN) [Vibrio fluvialis strain IDH05335]
MLHNINLIPWREAQREAHKRRFVGLCVLVLMVALGVQWGAGFYLDQQTQAQQQRLAFLNQHIRQLDAQIAELKVTEQEHK
ALLTRLNIVESLQQKRNKTTEFMNLMPQLIPEGVYVDKIKMNGEEIEISGISDSTARLATMLDNLEKSTQLSEVGMHSIV
SGNRRFGKQFQSFKVSFLFHASAHKPLATVTGEKHG

Nucleotide


Download         Length: 591 bp        

>NTDB_id=103957 QUF32_RS14350 WP_020329444.1 3043742..3044332(-) (pilN) [Vibrio fluvialis strain IDH05335]
ATGCTGCATAACATCAACCTTATTCCCTGGCGTGAAGCGCAGCGCGAGGCGCACAAACGCCGCTTCGTCGGACTGTGCGT
GCTCGTGTTGATGGTGGCATTGGGCGTGCAGTGGGGCGCTGGCTTTTATCTCGATCAGCAAACTCAGGCTCAGCAGCAGC
GACTGGCATTTCTCAATCAGCACATTCGCCAACTGGATGCGCAGATTGCCGAGCTGAAAGTGACCGAGCAAGAGCATAAA
GCGCTGCTGACCCGGCTCAATATCGTCGAATCGTTACAGCAAAAGCGCAATAAGACCACAGAGTTCATGAACCTGATGCC
GCAGTTGATTCCGGAAGGTGTCTACGTCGACAAGATCAAAATGAATGGCGAAGAAATTGAAATCAGCGGCATCAGTGACA
GTACTGCCCGACTCGCCACCATGCTCGACAATCTGGAAAAATCCACCCAACTGTCTGAGGTCGGTATGCATTCGATCGTG
TCAGGTAATCGACGTTTTGGTAAACAATTTCAGAGCTTTAAGGTGTCATTTCTGTTTCATGCGTCAGCTCATAAACCGCT
CGCCACCGTGACAGGAGAGAAACATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

69.149

95.918

0.663
  pilN Vibrio campbellii strain DS40M4

61.735

100

0.617

Multiple sequence alignment