Detailed information NTG0103958

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: QUF32_RS14355
• Genome accession: NZ_AP028128
• Coordinates: 3044322..3045326 (-)
• Length: 334 a.a.
• NCBI ID: WP_020329442.1
• Uniprot ID: S7I5K0
• Organism: Vibrio fluvialis strain IDH05335
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3039322..3050326

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QUF32_RS14330 (KKIDH5335_27510)	aroK	3040190..3040708 (-)	519	WP_004728569.1	shikimate kinase AroK	-
QUF32_RS14335 (KKIDH5335_27520)	pilQ	3040896..3042632 (-)	1737	WP_286236258.1	type IV pilus secretin PilQ family protein	Machinery gene
QUF32_RS14340 (KKIDH5335_27530)	pilP	3042657..3043169 (-)	513	WP_020329446.1	pilus assembly protein PilP	Machinery gene
QUF32_RS14345 (KKIDH5335_27540)	pilO	3043159..3043749 (-)	591	WP_020329445.1	type 4a pilus biogenesis protein PilO	Machinery gene
QUF32_RS14350 (KKIDH5335_27550)	pilN	3043742..3044332 (-)	591	WP_020329444.1	PilN domain-containing protein	Machinery gene
QUF32_RS14355 (KKIDH5335_27560)	pilM	3044322..3045326 (-)	1005	WP_020329442.1	type IV pilus assembly protein PilM	Machinery gene
QUF32_RS14360 (KKIDH5335_27570)	-	3045469..3047949 (+)	2481	WP_222733502.1	PBP1A family penicillin-binding protein	-
QUF32_RS14365 (KKIDH5335_27580)	oxyR	3048010..3048906 (-)	897	WP_020329438.1	DNA-binding transcriptional regulator OxyR	-
QUF32_RS14370 (KKIDH5335_27590)	-	3049050..3049784 (+)	735	WP_061057153.1	glutathione peroxidase	-
QUF32_RS14375 (KKIDH5335_27600)	-	3049913..3050176 (+)	264	WP_158125472.1	DUF3624 domain-containing protein	-

Sequence

Protein

• Length: 334 a.a.
• Molecular weight: 36847.20 Da
• Isoelectric Point: 6.6928

>NTDB_id=103958 QUF32_RS14355 WP_020329442.1 3044322..3045326(-) (pilM) [Vibrio fluvialis strain IDH05335]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSEDIFADNHTLNYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSELDASEQEFAVYQAFSHQSPLPIEELSLDFVVTGEKTLGRSSSLTYQVYATRREVIDNRLSAVSKAGFEP
LLFDMQIHGLLNIWQWVSRAQQRTDWMLVDVGFSQTSLCIDFADKTPFYKDIPLGTRQLVAATSGGNADLTCPQAHFIQD
LVERLARQIQLFLSLQGHQPLAGIWLSGGGAATEGLAQAISERLVLPCELFNPLAEFTCRASLRRQPQADLQRFSTAAGL
ALRGLNWLENGHAA

Nucleotide

• Length: 1005 bp

>NTDB_id=103958 QUF32_RS14355 WP_020329442.1 3044322..3045326(-) (pilM) [Vibrio fluvialis strain IDH05335]
ATGGGTAAATCATTAGTTACAGGTGTCGATATTGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAAGGCGAATC
GTATTCGCTGGTCGGTTACCAAGAGATAGTCGTATCCGAGGATATTTTCGCTGATAACCACACGCTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGCTATTTAGTCGTAAAGTGGCGCTGGCTGTACCGGACAACGCA
GTAATAAGTAAGGTATTACAGATAGATAGCGAACTGGATGCCAGCGAACAAGAGTTCGCGGTCTATCAGGCTTTTTCTCA
TCAATCGCCGCTACCCATCGAAGAGTTGAGCCTGGATTTCGTTGTCACCGGAGAAAAAACACTGGGGCGCAGCAGTTCGT
TGACGTATCAGGTCTATGCAACCCGACGTGAAGTGATCGACAACCGTTTGTCTGCGGTGAGCAAAGCGGGTTTTGAGCCG
CTGCTGTTTGATATGCAGATTCACGGCCTGCTCAATATCTGGCAATGGGTCAGCCGTGCTCAGCAACGCACCGATTGGAT
GCTGGTTGATGTCGGGTTCAGTCAAACCTCATTGTGCATCGACTTTGCCGATAAAACGCCGTTTTACAAAGACATTCCGT
TGGGAACCCGCCAACTGGTTGCGGCGACCTCCGGTGGTAACGCTGACCTTACCTGCCCGCAAGCACACTTCATTCAGGAT
TTGGTTGAGCGTCTGGCGCGCCAGATCCAGCTGTTTCTTTCTCTGCAAGGCCATCAGCCGCTGGCCGGTATCTGGCTATC
CGGTGGCGGCGCGGCAACGGAAGGTTTGGCGCAGGCGATTAGCGAACGTCTGGTTCTGCCTTGCGAGCTGTTTAACCCTC
TGGCGGAGTTTACTTGCCGCGCATCGCTACGCCGCCAGCCTCAGGCTGATTTGCAGCGTTTCAGTACTGCGGCCGGCTTG
GCGCTGCGCGGCCTGAATTGGCTGGAGAACGGCCATGCTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-219)

• Type IV pilus assembly protein PilM; (PilM_2) (237-324)

3D structure

• AlphaFold DB: S7I5K0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	65.487	100	0.665
pilM	Vibrio campbellii strain DS40M4	48.105	100	0.494