Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OC536_RS01495 Genome accession   NZ_OW443150
Coordinates   326205..326795 (+) Length   196 a.a.
NCBI ID   WP_000157690.1    Uniprot ID   A0A366AJW1
Organism   Vibrio cholerae strain CNRVC190247 isolate YE-NCPHL-18035-PI     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 321205..331795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OC536_RS01480 (CNRVC190247_00300) - 322002..324479 (-) 2478 WP_032477106.1 penicillin-binding protein 1A -
  OC536_RS01485 (CNRVC190247_00301) pilM 324622..325638 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  OC536_RS01490 (CNRVC190247_00302) pilN 325628..326212 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  OC536_RS01495 (CNRVC190247_00303) pilO 326205..326795 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  OC536_RS01500 (CNRVC190247_00304) pilP 326785..327303 (+) 519 WP_172779491.1 pilus assembly protein PilP Machinery gene
  OC536_RS01505 (CNRVC190247_00305) pilQ 327329..329044 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  OC536_RS01510 (CNRVC190247_00306) aroK 329231..329755 (+) 525 WP_000818616.1 shikimate kinase AroK -
  OC536_RS01515 (CNRVC190247_00307) aroB 329783..330868 (+) 1086 WP_000439805.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22877.27 Da        Isoelectric Point: 4.4169

>NTDB_id=1036013 OC536_RS01495 WP_000157690.1 326205..326795(+) (pilO) [Vibrio cholerae strain CNRVC190247 isolate YE-NCPHL-18035-PI]
MASLQELELDEISEWPLLPQLVVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=1036013 OC536_RS01495 WP_000157690.1 326205..326795(+) (pilO) [Vibrio cholerae strain CNRVC190247 isolate YE-NCPHL-18035-PI]
ATGGCTAGTTTGCAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGTTGGTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCCACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCCCAGTTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACTGGAAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGGGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTTAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366AJW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

100

100

1
  pilO Vibrio campbellii strain DS40M4

71.958

96.429

0.694