Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACA109_RS05035 Genome accession   NZ_CP166872
Coordinates   955802..956299 (+) Length   165 a.a.
NCBI ID   WP_029693246.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 24EBSta0529     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 950802..961299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA109_RS04995 (ACA109_04995) - 950846..951832 (+) 987 WP_000161315.1 ROK family glucokinase -
  ACA109_RS05000 (ACA109_05000) - 951832..952161 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACA109_RS05005 (ACA109_05005) - 952158..952781 (+) 624 WP_371257211.1 MBL fold metallo-hydrolase -
  ACA109_RS05010 (ACA109_05010) comGA 952833..953807 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACA109_RS05015 (ACA109_05015) comGB 953779..954849 (+) 1071 WP_371257212.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACA109_RS05020 (ACA109_05020) comGC 954863..955174 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACA109_RS05025 (ACA109_05025) comGD 955152..955598 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACA109_RS05030 (ACA109_05030) comGE 955585..955884 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACA109_RS05035 (ACA109_05035) comGF 955802..956299 (+) 498 WP_029693246.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACA109_RS05040 (ACA109_05040) - 956396..956542 (+) 147 WP_001789879.1 hypothetical protein -
  ACA109_RS05045 (ACA109_05045) - 956532..957056 (+) 525 WP_371257213.1 shikimate kinase -
  ACA109_RS05050 (ACA109_05050) gcvT 957215..958306 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ACA109_RS05055 (ACA109_05055) gcvPA 958326..959672 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACA109_RS05060 (ACA109_05060) gcvPB 959665..961137 (+) 1473 WP_371257214.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2192

>NTDB_id=1035839 ACA109_RS05035 WP_029693246.1 955802..956299(+) (comGF) [Staphylococcus aureus strain 24EBSta0529]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMLNNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1035839 ACA109_RS05035 WP_029693246.1 955802..956299(+) (comGF) [Staphylococcus aureus strain 24EBSta0529]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGCTTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

97.576

100

0.976
  comGF Staphylococcus aureus N315

97.576

100

0.976

Multiple sequence alignment