Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ACA109_RS05015 Genome accession   NZ_CP166872
Coordinates   953779..954849 (+) Length   356 a.a.
NCBI ID   WP_371257212.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 24EBSta0529     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 948779..959849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA109_RS04985 (ACA109_04985) - 949202..950665 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  ACA109_RS04990 (ACA109_04990) - 950646..950849 (+) 204 WP_000087561.1 YqgQ family protein -
  ACA109_RS04995 (ACA109_04995) - 950846..951832 (+) 987 WP_000161315.1 ROK family glucokinase -
  ACA109_RS05000 (ACA109_05000) - 951832..952161 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACA109_RS05005 (ACA109_05005) - 952158..952781 (+) 624 WP_371257211.1 MBL fold metallo-hydrolase -
  ACA109_RS05010 (ACA109_05010) comGA 952833..953807 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACA109_RS05015 (ACA109_05015) comGB 953779..954849 (+) 1071 WP_371257212.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACA109_RS05020 (ACA109_05020) comGC 954863..955174 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACA109_RS05025 (ACA109_05025) comGD 955152..955598 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACA109_RS05030 (ACA109_05030) comGE 955585..955884 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACA109_RS05035 (ACA109_05035) comGF 955802..956299 (+) 498 WP_029693246.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACA109_RS05040 (ACA109_05040) - 956396..956542 (+) 147 WP_001789879.1 hypothetical protein -
  ACA109_RS05045 (ACA109_05045) - 956532..957056 (+) 525 WP_371257213.1 shikimate kinase -
  ACA109_RS05050 (ACA109_05050) gcvT 957215..958306 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ACA109_RS05055 (ACA109_05055) gcvPA 958326..959672 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41479.93 Da        Isoelectric Point: 10.1030

>NTDB_id=1035835 ACA109_RS05015 WP_371257212.1 953779..954849(+) (comGB) [Staphylococcus aureus strain 24EBSta0529]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMAYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIILLNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKFIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLIIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1035835 ACA109_RS05015 WP_371257212.1 953779..954849(+) (comGB) [Staphylococcus aureus strain 24EBSta0529]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGGCATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATTTTTA
TTGCTATGATTATTCTATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTTATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTTATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTTGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAG
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAATAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

98.596

100

0.986
  comGB Staphylococcus aureus N315

98.596

100

0.986

Multiple sequence alignment