Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ABKQ09_RS12475 Genome accession   NZ_CP166870
Coordinates   2514132..2515202 (-) Length   356 a.a.
NCBI ID   WP_063638548.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain F4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2509132..2520202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABKQ09_RS12430 (ABKQ09_12425) tapA 2509667..2510368 (-) 702 WP_371175996.1 amyloid fiber anchoring/assembly protein TapA -
  ABKQ09_RS12435 (ABKQ09_12430) - 2510630..2510953 (+) 324 WP_003325436.1 DUF3889 domain-containing protein -
  ABKQ09_RS12440 (ABKQ09_12435) - 2511000..2511179 (-) 180 WP_003325435.1 YqzE family protein -
  ABKQ09_RS12445 (ABKQ09_12440) comGG 2511249..2511623 (-) 375 WP_061670002.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABKQ09_RS12450 (ABKQ09_12445) comGF 2511624..2512130 (-) 507 WP_326938418.1 competence type IV pilus minor pilin ComGF -
  ABKQ09_RS12455 (ABKQ09_12450) comGE 2512033..2512380 (-) 348 WP_003325432.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABKQ09_RS12460 (ABKQ09_12455) comGD 2512364..2512804 (-) 441 WP_061670000.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABKQ09_RS12465 (ABKQ09_12460) comGC 2512794..2513093 (-) 300 WP_003325429.1 competence type IV pilus major pilin ComGC Machinery gene
  ABKQ09_RS12470 (ABKQ09_12465) comGB 2513108..2514145 (-) 1038 WP_268481127.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABKQ09_RS12475 (ABKQ09_12470) comGA 2514132..2515202 (-) 1071 WP_063638548.1 competence type IV pilus ATPase ComGA Machinery gene
  ABKQ09_RS12480 (ABKQ09_12475) - 2515619..2516572 (-) 954 WP_003325425.1 magnesium transporter CorA family protein -
  ABKQ09_RS12485 (ABKQ09_12480) - 2516694..2518042 (+) 1349 Protein_2419 hemolysin family protein -
  ABKQ09_RS12490 (ABKQ09_12485) - 2518118..2518969 (-) 852 WP_371175997.1 STAS domain-containing protein -
  ABKQ09_RS12500 (ABKQ09_12495) - 2519228..2519608 (-) 381 WP_003325422.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ABKQ09_RS12505 (ABKQ09_12500) - 2519846..2520091 (+) 246 WP_003325421.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40457.76 Da        Isoelectric Point: 9.4624

>NTDB_id=1035764 ABKQ09_RS12475 WP_063638548.1 2514132..2515202(-) (comGA) [Bacillus atrophaeus strain F4]
MDTIEKTSRTLIEEAYSVKASDIHIVPREQDALIHFRIDHALLKKRTMKKEECVRLISHFKFLSAMDIGERRKPQSGSLS
LKLKKGTVYLRMSTLPTINDESLVIRVLPQHNIPAIERLSLFPKTGATLLSFLKHSHGLIIFTGPTGSGKSTTLYSLVQY
AKKHFNRNIITLEDPVETRDQDVLQVQVNEKAGITYSAGLKAILRHDPDMIVLGEIRDAETAKIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGVNMNEIEQTAIAIAAQRLVDLTCPFCENGCSSVYCRITRDVRRATIYELLYGKNLQQCIQEARGE
RANYQYRTLRQIIRKGIALGYLTTNNYDRWVYHEED

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1035764 ABKQ09_RS12475 WP_063638548.1 2514132..2515202(-) (comGA) [Bacillus atrophaeus strain F4]
TTGGATACGATAGAAAAAACTAGCAGAACATTAATTGAAGAGGCATATTCCGTGAAGGCCTCGGATATTCACATTGTACC
AAGGGAGCAAGATGCTTTGATCCATTTTCGGATTGACCATGCGTTGCTGAAGAAGCGGACAATGAAAAAGGAAGAGTGCG
TTCGGCTCATTTCCCATTTTAAATTTCTGTCAGCCATGGATATAGGAGAAAGGCGGAAGCCACAAAGTGGTTCACTTTCT
TTAAAGCTTAAAAAAGGGACAGTTTATCTCAGAATGTCGACACTTCCTACGATTAATGATGAAAGCCTCGTGATCAGAGT
TTTGCCTCAGCATAATATTCCTGCGATCGAAAGACTGTCCCTGTTCCCAAAGACAGGCGCCACGCTGCTATCCTTTTTAA
AGCACTCCCACGGCCTCATTATTTTTACCGGTCCGACTGGCTCAGGGAAATCTACCACCCTATATTCACTGGTTCAATAT
GCAAAAAAGCATTTCAACCGAAATATTATCACTCTCGAAGATCCTGTGGAAACAAGAGATCAGGATGTCTTGCAGGTACA
GGTGAATGAAAAAGCCGGCATTACGTACTCAGCGGGCCTGAAGGCGATTTTGCGCCATGATCCAGACATGATTGTTCTGG
GGGAAATCAGAGATGCTGAAACAGCAAAAATCGCCGTGCGGGCTGCTATGACAGGCCATTTGGTATTAACAAGCCTGCAC
ACAAGAGATGCCAAGGGCGCCATTTACAGACTGCTTGAATTCGGAGTAAATATGAATGAAATAGAGCAAACAGCGATTGC
GATAGCAGCCCAGCGATTAGTCGACTTAACCTGTCCATTTTGTGAAAACGGCTGTTCCTCTGTTTATTGCCGGATTACAC
GTGATGTAAGGCGAGCGACCATTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATACAAGAAGCTAGAGGAGAA
CGGGCCAATTATCAGTACCGAACCTTACGGCAGATTATCAGAAAAGGAATCGCGCTTGGCTATCTAACGACGAATAATTA
CGACCGGTGGGTTTATCATGAAGAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

87.921

100

0.879

Multiple sequence alignment