Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OAM_RS15140 Genome accession   NZ_CP166824
Coordinates   3353818..3354447 (-) Length   209 a.a.
NCBI ID   WP_010435284.1    Uniprot ID   A0AAN0LMD0
Organism   Vibrio cyclitrophicus ZF14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3348818..3359447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OAM_RS15120 (OAM_015120) aroB 3349754..3350842 (-) 1089 WP_010435273.1 3-dehydroquinate synthase -
  OAM_RS15125 (OAM_015125) aroK 3350867..3351385 (-) 519 WP_009848907.1 shikimate kinase AroK -
  OAM_RS15130 (OAM_015130) pilQ 3351562..3353253 (-) 1692 WP_010435279.1 type IV pilus secretin PilQ Machinery gene
  OAM_RS15135 (OAM_015135) pilP 3353313..3353831 (-) 519 WP_010435282.1 pilus assembly protein PilP Machinery gene
  OAM_RS15140 (OAM_015140) pilO 3353818..3354447 (-) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  OAM_RS15145 (OAM_015145) pilN 3354440..3355054 (-) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  OAM_RS15150 (OAM_015150) pilM 3355038..3356063 (-) 1026 WP_010435291.1 type IV pilus assembly protein PilM Machinery gene
  OAM_RS15155 (OAM_015155) - 3356215..3358773 (+) 2559 WP_010435294.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24324.99 Da        Isoelectric Point: 4.9074

>NTDB_id=1035459 OAM_RS15140 WP_010435284.1 3353818..3354447(-) (pilO) [Vibrio cyclitrophicus ZF14]
MANLQNRISWQNRMSLQDLDVDEITEWPLLPQLLVILLLMVLIQGLGTWLYILPLDDELQKMKQQEQTLKATLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGQKQNKAFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=1035459 OAM_RS15140 WP_010435284.1 3353818..3354447(-) (pilO) [Vibrio cyclitrophicus ZF14]
ATGGCTAACCTTCAAAATAGGATTAGTTGGCAAAATAGGATGAGCTTGCAAGATCTTGATGTTGATGAAATCACCGAATG
GCCGTTACTGCCTCAATTACTGGTGATTCTGTTGTTGATGGTGCTTATCCAAGGATTAGGGACTTGGCTCTATATTCTGC
CTTTGGACGATGAGCTACAGAAAATGAAACAACAAGAGCAAACCTTAAAAGCGACCTTGAGAATAAAGGCCAATAAGGTT
GCAGCCTTGCCTAAGTTGCAGAGTCAGTTAGATGAATTAACTAGCCGTTACGACTACTTGTTAGAGCAACTACCGGTTCA
AAAGGAGCTGGCCAGCATGTTGGCCTCAGTGAATGAACTTGGCTTGGATAACAAGCTGACGTTTACACGGATTGATTGGG
GGCAGAAGCAAAACAAAGCGTTTCTGTATCGGTTACCTCTCAATATCGAGTTGACGGGTGACTATCATGAGATTGGTGAT
TTCTCGGCGGCCATCGCTAAGCTGCCACGAATTATTAGCTTTGATGATGTGAACTGGCAGCGAGTCAGTCAAGAAAGTAG
CACGCTGCACTTTAGGGTTCGCGCTTATACCTACCAGTTTAAGTCGGAGGTGAATGATGAAACCCAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

76.684

92.344

0.708
  pilO Vibrio campbellii strain DS40M4

74.054

88.517

0.656

Multiple sequence alignment