Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OAM_RS15150 Genome accession   NZ_CP166824
Coordinates   3355038..3356063 (-) Length   341 a.a.
NCBI ID   WP_010435291.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus ZF14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3350038..3361063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OAM_RS15125 (OAM_015125) aroK 3350867..3351385 (-) 519 WP_009848907.1 shikimate kinase AroK -
  OAM_RS15130 (OAM_015130) pilQ 3351562..3353253 (-) 1692 WP_010435279.1 type IV pilus secretin PilQ Machinery gene
  OAM_RS15135 (OAM_015135) pilP 3353313..3353831 (-) 519 WP_010435282.1 pilus assembly protein PilP Machinery gene
  OAM_RS15140 (OAM_015140) pilO 3353818..3354447 (-) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  OAM_RS15145 (OAM_015145) pilN 3354440..3355054 (-) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  OAM_RS15150 (OAM_015150) pilM 3355038..3356063 (-) 1026 WP_010435291.1 type IV pilus assembly protein PilM Machinery gene
  OAM_RS15155 (OAM_015155) - 3356215..3358773 (+) 2559 WP_010435294.1 penicillin-binding protein 1A -
  OAM_RS15160 (OAM_015160) oxyR 3358869..3359762 (-) 894 WP_010435297.1 DNA-binding transcriptional regulator OxyR -
  OAM_RS15165 (OAM_015165) - 3359984..3360712 (+) 729 WP_010435300.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38098.75 Da        Isoelectric Point: 6.8348

>NTDB_id=1035461 OAM_RS15150 WP_010435291.1 3355038..3356063(-) (pilM) [Vibrio cyclitrophicus ZF14]
MGSSLITGIDINHHSIKAVVLKPVGESYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGATSSYQVYATRKEVVDSRADGLTKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGVDQTSLGIIPQGSAPFYKDIAFGIQDLCSTGNSMDMEGGFSNVEEKH
KFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQLSCESLNPLSLFENKVAKKRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=1035461 OAM_RS15150 WP_010435291.1 3355038..3356063(-) (pilM) [Vibrio cyclitrophicus ZF14]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCCGTGGTACTAAAACCCGTTGGGGAGTC
GTATGCCCTGGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTTGGAAGCCGCAACGTCGCTATTTCGGTACCGGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGCGAATTAGAAGATAGAGAAAAGGAGTTCTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGAGCAACAA
GCAGCTATCAAGTGTATGCGACTCGTAAAGAGGTGGTTGATAGCCGAGCTGACGGGCTAACGAAAGCAGGGTTTAAGCCC
ATCGTTGTCGACACTCAGGCCCATGGTTTACTCAACATTTGGCAATTGGCTTCGCATTTGTATCCAGAAAAAAAGAATTG
GCTGTTGGTGGATGTTGGCGTTGATCAAACTTCATTAGGCATTATTCCCCAAGGTTCAGCGCCATTTTATAAAGATATTG
CCTTTGGCATTCAAGATCTTTGCAGTACGGGTAACTCGATGGATATGGAGGGTGGGTTTAGTAACGTTGAGGAGAAGCAT
AAGTTTATTGTCAATTTGATTGAAAAACTCAAGCGTCAGTTACAGCTCTATTCATCGGTCAATGCGCTTCAACCTATCTC
AGGAATTTGGCTAATGGGCGAAGGAGCAAGTATGCCGATGGTCGTTGAAGAGTTAGAGCGACATTTTCAGTTAAGTTGTG
AGTCTTTGAACCCCCTATCTTTGTTTGAGAATAAGGTCGCGAAAAAACGTCGATTACCCATGGATTGGCAACATTTTGGT
ATTGCAGCAGGCATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

52.802

99.413

0.525
  pilM Vibrio campbellii strain DS40M4

47.368

100

0.475

Multiple sequence alignment