Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JNUCC21_RS13145 Genome accession   NZ_CP166019
Coordinates   2642999..2644069 (-) Length   356 a.a.
NCBI ID   WP_007408320.1    Uniprot ID   -
Organism   Bacillus sp. JNUCC-21     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2637999..2649069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNUCC21_RS13095 (JNUCC21_13125) sipW 2638058..2638642 (-) 585 WP_015240205.1 signal peptidase I SipW -
  JNUCC21_RS13100 (JNUCC21_13130) tapA 2638614..2639285 (-) 672 WP_150941310.1 amyloid fiber anchoring/assembly protein TapA -
  JNUCC21_RS13105 (JNUCC21_13135) - 2639544..2639873 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  JNUCC21_RS13110 (JNUCC21_13140) - 2639914..2640093 (-) 180 WP_003153093.1 YqzE family protein -
  JNUCC21_RS13115 (JNUCC21_13145) comGG 2640150..2640527 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  JNUCC21_RS13120 (JNUCC21_13150) comGF 2640528..2641028 (-) 501 WP_257474763.1 competence type IV pilus minor pilin ComGF -
  JNUCC21_RS13125 (JNUCC21_13155) comGE 2640937..2641251 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  JNUCC21_RS13130 (JNUCC21_13160) comGD 2641235..2641672 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  JNUCC21_RS13135 (JNUCC21_13165) comGC 2641662..2641970 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  JNUCC21_RS13140 (JNUCC21_13170) comGB 2641975..2643012 (-) 1038 WP_218791295.1 competence type IV pilus assembly protein ComGB Machinery gene
  JNUCC21_RS13145 (JNUCC21_13175) comGA 2642999..2644069 (-) 1071 WP_007408320.1 competence type IV pilus ATPase ComGA Machinery gene
  JNUCC21_RS13150 (JNUCC21_13180) - 2644262..2645212 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  JNUCC21_RS13155 (JNUCC21_13185) - 2645358..2646659 (+) 1302 WP_369878206.1 hemolysin family protein -
  JNUCC21_RS13160 (JNUCC21_13190) - 2646697..2647527 (-) 831 WP_029326073.1 STAS domain-containing protein -
  JNUCC21_RS13170 (JNUCC21_13200) - 2647734..2648114 (-) 381 WP_038459192.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  JNUCC21_RS13175 (JNUCC21_13205) - 2648351..2648596 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40530.79 Da        Isoelectric Point: 9.1996

>NTDB_id=1033129 JNUCC21_RS13145 WP_007408320.1 2642999..2644069(-) (comGA) [Bacillus sp. JNUCC-21]
MENIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1033129 JNUCC21_RS13145 WP_007408320.1 2642999..2644069(-) (comGA) [Bacillus sp. JNUCC-21]
GTGGAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGTCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATTGCACTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment