Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB8E30_RS07045 Genome accession   NZ_CP165965
Coordinates   1346422..1347465 (+) Length   347 a.a.
NCBI ID   WP_369902626.1    Uniprot ID   -
Organism   Bacillus manliponensis strain jeju3-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1341422..1352465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E30_RS07015 (AB8E30_07020) - 1341499..1341993 (+) 495 WP_369902620.1 hypothetical protein -
  AB8E30_RS07020 (AB8E30_07025) - 1342217..1342432 (+) 216 WP_369902621.1 DUF3912 family protein -
  AB8E30_RS07025 (AB8E30_07030) - 1342481..1343872 (-) 1392 WP_369902622.1 L-cystine transporter -
  AB8E30_RS07030 (AB8E30_07035) - 1344079..1344645 (-) 567 WP_369902623.1 hypothetical protein -
  AB8E30_RS07035 (AB8E30_07040) - 1344803..1345045 (-) 243 WP_369902624.1 DUF2626 domain-containing protein -
  AB8E30_RS07040 (AB8E30_07045) - 1345498..1346193 (-) 696 WP_369902625.1 helix-turn-helix transcriptional regulator -
  AB8E30_RS07045 (AB8E30_07050) comGA 1346422..1347465 (+) 1044 WP_369902626.1 competence type IV pilus ATPase ComGA Machinery gene
  AB8E30_RS07050 (AB8E30_07055) comGB 1347458..1348492 (+) 1035 WP_369902627.1 competence type IV pilus assembly protein ComGB -
  AB8E30_RS07055 (AB8E30_07060) comGC 1348505..1348810 (+) 306 WP_369902629.1 competence type IV pilus major pilin ComGC -
  AB8E30_RS07060 (AB8E30_07065) comGD 1348807..1349241 (+) 435 WP_369902630.1 competence type IV pilus minor pilin ComGD -
  AB8E30_RS07065 (AB8E30_07070) - 1349231..1349548 (+) 318 WP_369902631.1 type II secretion system protein -
  AB8E30_RS07070 (AB8E30_07075) comGF 1349535..1349972 (+) 438 WP_369902632.1 competence type IV pilus minor pilin ComGF -
  AB8E30_RS07075 (AB8E30_07080) comGG 1349969..1350346 (+) 378 WP_369902633.1 competence type IV pilus minor pilin ComGG -
  AB8E30_RS07080 (AB8E30_07085) - 1350348..1350845 (+) 498 WP_369902634.1 shikimate kinase -
  AB8E30_RS07085 (AB8E30_07090) - 1350883..1351083 (+) 201 WP_369902635.1 YqzE family protein -
  AB8E30_RS07090 (AB8E30_07095) - 1351759..1351986 (+) 228 WP_369902636.1 hypothetical protein -
  AB8E30_RS07095 (AB8E30_07100) - 1352080..1352226 (+) 147 WP_200874674.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39515.26 Da        Isoelectric Point: 9.4979

>NTDB_id=1032635 AB8E30_RS07045 WP_369902626.1 1346422..1347465(+) (comGA) [Bacillus manliponensis strain jeju3-1]
MKSVERFADNLVKEACMLRASDIHIIPRHEEAVVQFRIDRDLLTKYYIAKEFGERLVSHFKFLASMDIGERRKPQNGALQ
MQIKEADVYLRLSTLPTIYYESLVIRIHLQGSTEHLSHLSLFPNTATKLLSFLHHSHGLLIFTGPTGSGKTTTIYSLLEV
AKKQGLRRIVTLEDPVEKRKEGLLQIQINEKAGITYAAGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVLTTLH
TNDARGAVLRLLDFGVTLQEIEQALLAVAAQRLVELKCPFCQKRCSPLCKKMRKVRRASIYEVLYRNELKKVIREAQGEK
VTYCYMTLQDCIRKGKALGFLEEDMYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1032635 AB8E30_RS07045 WP_369902626.1 1346422..1347465(+) (comGA) [Bacillus manliponensis strain jeju3-1]
ATGAAGAGTGTCGAACGGTTTGCAGATAATTTAGTGAAAGAAGCATGTATGTTACGAGCATCAGATATCCATATTATTCC
GCGTCATGAAGAAGCAGTTGTGCAGTTTAGAATTGACAGAGATTTATTGACAAAGTATTACATCGCAAAGGAATTTGGAG
AACGCCTTGTGTCACATTTTAAATTTCTAGCTTCAATGGATATTGGAGAAAGGAGAAAACCACAAAATGGGGCACTACAG
ATGCAAATAAAAGAAGCAGATGTATATTTACGATTATCAACGTTACCAACTATTTATTATGAAAGTCTTGTTATTCGAAT
TCATCTCCAAGGCTCAACGGAGCACCTCTCTCACCTTTCATTATTTCCAAATACCGCTACAAAACTTCTCTCCTTTTTAC
ATCATTCCCACGGTTTATTAATATTTACAGGCCCAACTGGTTCTGGTAAAACAACAACGATTTATTCTCTATTAGAAGTA
GCAAAAAAACAAGGATTACGCCGTATTGTAACCCTCGAAGATCCAGTAGAGAAAAGAAAAGAAGGTTTATTACAGATTCA
AATTAATGAAAAAGCAGGAATTACGTATGCAGCAGGATTAAAAGCTATTTTACGACATGATCCAGATATTATTTTAGTTG
GAGAAATTAGAGATGAGGAAACCGCTAAAATAGCAATTCGGGCAAGTTTAACAGGTCATTTAGTGTTAACGACGTTGCAT
ACGAACGATGCAAGGGGAGCAGTGCTACGCCTGTTAGACTTCGGTGTTACACTTCAAGAAATCGAACAAGCATTGTTGGC
AGTTGCTGCGCAAAGGCTTGTTGAGTTGAAATGTCCGTTTTGTCAAAAGAGGTGTTCTCCTTTATGTAAGAAAATGAGGA
AAGTGCGCCGAGCTAGTATTTATGAGGTGTTATATAGAAATGAGCTGAAAAAAGTAATTCGTGAGGCACAAGGTGAAAAG
GTAACGTATTGTTATATGACTTTGCAAGATTGTATCCGAAAAGGAAAAGCTTTAGGTTTTTTAGAAGAGGATATGTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

60.519

100

0.605
  pilB Glaesserella parasuis strain SC1401

44

86.455

0.38
  pilB Haemophilus influenzae 86-028NP

40

95.101

0.38
  pilB Haemophilus influenzae Rd KW20

39.514

94.813

0.375

Multiple sequence alignment