Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PANN_RS30050 Genome accession   NZ_LT883143
Coordinates   6308116..6308739 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa C-NN2 isolate early isolate NN2 (clone C)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6303116..6313739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PANN_RS30030 (PANN_60190) aroB 6303711..6304817 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  PANN_RS30035 (PANN_60200) aroK 6304867..6305385 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PANN_RS30040 (PANN_60210) pilQ 6305397..6307541 (-) 2145 WP_023087871.1 type 4a pilus secretin PilQ Machinery gene
  PANN_RS30045 (PANN_60220) comL/pilP 6307595..6308119 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PANN_RS30050 (PANN_60230) pilO 6308116..6308739 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PANN_RS30055 (PANN_60240) pilN 6308736..6309332 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PANN_RS30060 (PANN_60250) pilM 6309332..6310396 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PANN_RS30065 (PANN_60260) - 6310581..6313052 (+) 2472 WP_016562439.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=1031293 PANN_RS30050 WP_003103268.1 6308116..6308739(-) (pilO) [Pseudomonas aeruginosa C-NN2 isolate early isolate NN2 (clone C)]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=1031293 PANN_RS30050 WP_003103268.1 6308116..6308739(-) (pilO) [Pseudomonas aeruginosa C-NN2 isolate early isolate NN2 (clone C)]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGTGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAATGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401

Multiple sequence alignment