Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB3D65_RS04330 Genome accession   NZ_CP165606
Coordinates   768650..769720 (+) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain BP1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 763650..774720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3D65_RS04300 (AB3D65_04300) - 764123..764368 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  AB3D65_RS04305 (AB3D65_04305) - 764605..764985 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AB3D65_RS04315 (AB3D65_04315) - 765192..766022 (+) 831 WP_007408317.1 STAS domain-containing protein -
  AB3D65_RS04320 (AB3D65_04320) - 766060..767361 (-) 1302 WP_369546433.1 hemolysin family protein -
  AB3D65_RS04325 (AB3D65_04325) - 767507..768457 (+) 951 WP_007408319.1 magnesium transporter CorA family protein -
  AB3D65_RS04330 (AB3D65_04330) comGA 768650..769720 (+) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  AB3D65_RS04335 (AB3D65_04335) comGB 769707..770744 (+) 1038 WP_369546434.1 competence type IV pilus assembly protein ComGB Machinery gene
  AB3D65_RS04340 (AB3D65_04340) comGC 770749..771057 (+) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  AB3D65_RS04345 (AB3D65_04345) comGD 771047..771484 (+) 438 WP_369546435.1 competence type IV pilus minor pilin ComGD Machinery gene
  AB3D65_RS04350 (AB3D65_04350) comGE 771468..771782 (+) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  AB3D65_RS04355 (AB3D65_04355) comGF 771691..772191 (+) 501 WP_369546436.1 competence type IV pilus minor pilin ComGF -
  AB3D65_RS04360 (AB3D65_04360) comGG 772192..772569 (+) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  AB3D65_RS04365 (AB3D65_04365) - 772626..772805 (+) 180 WP_003153093.1 YqzE family protein -
  AB3D65_RS04370 (AB3D65_04370) - 772845..773174 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  AB3D65_RS04375 (AB3D65_04375) tapA 773433..774104 (+) 672 WP_151296368.1 amyloid fiber anchoring/assembly protein TapA -
  AB3D65_RS04380 (AB3D65_04380) sipW 774076..774660 (+) 585 WP_012117977.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=1030784 AB3D65_RS04330 WP_012117985.1 768650..769720(+) (comGA) [Bacillus velezensis strain BP1]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1030784 AB3D65_RS04330 WP_012117985.1 768650..769720(+) (comGA) [Bacillus velezensis strain BP1]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGACTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACATCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGTAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment