Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   BQ8897_RS01455 Genome accession   NZ_LT714196
Coordinates   236550..236978 (+) Length   142 a.a.
NCBI ID   WP_223842274.1    Uniprot ID   -
Organism   Streptococcus agalactiae isolate BM110     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 231550..241978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BQ8897_RS01435 (BQ8897_BM110_00279) - 234080..234445 (+) 366 WP_000285373.1 DUF1033 family protein -
  BQ8897_RS01445 (BQ8897_BM110_00280) comGA 234617..235588 (+) 972 WP_000250797.1 competence type IV pilus ATPase ComGA Machinery gene
  BQ8897_RS01450 (BQ8897_BM110_00281) comGB 235482..236525 (+) 1044 WP_223297804.1 competence type IV pilus assembly protein ComGB Machinery gene
  BQ8897_RS01455 (BQ8897_BM110_00283) comGF 236550..236978 (+) 429 WP_223842274.1 competence type IV pilus minor pilin ComGF Machinery gene
  BQ8897_RS01460 (BQ8897_BM110_00284) comGG 236956..237179 (+) 224 Protein_237 competence type IV pilus minor pilin ComGG -
  BQ8897_RS01465 (BQ8897_BM110_00285) comGH 237294..238268 (+) 975 WP_001008571.1 class I SAM-dependent methyltransferase Machinery gene
  BQ8897_RS01470 (BQ8897_BM110_00286) - 238300..239493 (+) 1194 WP_000047534.1 acetate kinase -
  BQ8897_RS01475 (BQ8897_BM110_00287) - 239644..239850 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  BQ8897_RS01480 - 239909..240046 (+) 138 WP_001869149.1 hypothetical protein -
  BQ8897_RS01485 (BQ8897_BM110_00288) - 240087..240542 (+) 456 WP_000905672.1 hypothetical protein -
  BQ8897_RS01490 (BQ8897_BM110_00289) - 240611..241276 (+) 666 WP_000008107.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 16590.29 Da        Isoelectric Point: 9.7864

>NTDB_id=1030632 BQ8897_RS01455 WP_223842274.1 236550..236978(+) (comGF) [Streptococcus agalactiae isolate BM110]
MRRLKHLHILECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKI
VTFGKSNKDDFRKTGYNGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide


Download         Length: 429 bp        

>NTDB_id=1030632 BQ8897_RS01455 WP_223842274.1 236550..236978(+) (comGF) [Streptococcus agalactiae isolate BM110]
ATAAGAAGGTTAAAGCATTTACACATTTTAGAATGTTTGGTAGCATTGGTTACAATCACAGGAGCTTTGCTAGTTTATCA
AGGACTAACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTCAGTCTGAATGGGTGTTATTAACTCAGC
AACTAAATGCAGAATTCGAAGGCGCTCACTTGGAATATTTAAGACAGAACAAACTTTATTTACGTAAGCAAGATAAGATT
GTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATAATGGTCGAGGTTATCAACCAATGGTTTATGG
GTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTGTTTTTTATTTTAAGGACGGGTTAAAAAGGACAT
TTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(38-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA159

44.355

87.324

0.387
  comGF Streptococcus mutans UA140

44.355

87.324

0.387
  comGF/cglF Streptococcus pneumoniae D39

39.568

97.887

0.387
  comGF/cglF Streptococcus pneumoniae TIGR4

39.568

97.887

0.387
  comGF/cglF Streptococcus pneumoniae R6

39.568

97.887

0.387
  comGF/cglF Streptococcus pneumoniae Rx1

39.568

97.887

0.387
  comGF/cglF Streptococcus mitis NCTC 12261

40.299

94.366

0.38
  comGF/cglF Streptococcus mitis SK321

40.299

94.366

0.38

Multiple sequence alignment