Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   AB6M99_RS10170 Genome accession   NZ_CP163513
Coordinates   2067268..2067723 (-) Length   151 a.a.
NCBI ID   WP_121835590.1    Uniprot ID   -
Organism   Streptococcus hillyeri strain S23-3001-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2068659..2107455 2067268..2067723 flank 936


Gene organization within MGE regions


Location: 2067268..2107455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB6M99_RS10170 (AB6M99_10170) comYF 2067268..2067723 (-) 456 WP_121835590.1 competence type IV pilus minor pilin ComGF Machinery gene
  AB6M99_RS10175 (AB6M99_10175) comGE 2067689..2067983 (-) 295 Protein_1949 competence type IV pilus minor pilin ComGE -
  AB6M99_RS10180 (AB6M99_10180) comYD 2067955..2068362 (-) 408 WP_121835591.1 competence type IV pilus minor pilin ComGD Machinery gene
  AB6M99_RS10185 (AB6M99_10185) - 2068659..2070074 (-) 1416 WP_121835595.1 peptidoglycan amidohydrolase family protein -
  AB6M99_RS10190 (AB6M99_10190) - 2070203..2070430 (-) 228 WP_121835597.1 phage holin -
  AB6M99_RS10195 (AB6M99_10195) - 2070427..2070732 (-) 306 WP_121835599.1 hypothetical protein -
  AB6M99_RS10200 (AB6M99_10200) - 2070736..2070876 (-) 141 WP_183121663.1 hypothetical protein -
  AB6M99_RS10205 (AB6M99_10205) - 2070908..2071363 (-) 456 WP_121835603.1 DUF1366 domain-containing protein -
  AB6M99_RS10210 (AB6M99_10210) - 2071360..2071545 (-) 186 WP_121835605.1 hypothetical protein -
  AB6M99_RS10215 (AB6M99_10215) - 2071555..2076792 (-) 5238 WP_121835607.1 phage tail spike protein -
  AB6M99_RS10220 (AB6M99_10220) - 2076789..2077580 (-) 792 WP_121835609.1 distal tail protein Dit -
  AB6M99_RS10225 (AB6M99_10225) - 2077592..2080969 (-) 3378 WP_369350721.1 phage tail tape measure protein -
  AB6M99_RS10230 (AB6M99_10230) - 2080988..2081251 (-) 264 WP_121835613.1 hypothetical protein -
  AB6M99_RS10235 (AB6M99_10235) - 2081317..2081661 (-) 345 WP_121835615.1 tail assembly chaperone -
  AB6M99_RS10240 (AB6M99_10240) - 2081714..2082244 (-) 531 WP_121835617.1 phage major tail protein, TP901-1 family -
  AB6M99_RS10245 (AB6M99_10245) - 2082259..2082645 (-) 387 WP_121835619.1 phage capsid protein -
  AB6M99_RS10250 (AB6M99_10250) - 2082642..2083037 (-) 396 WP_220702229.1 HK97-gp10 family putative phage morphogenesis protein -
  AB6M99_RS10255 (AB6M99_10255) - 2083030..2083335 (-) 306 WP_121835623.1 hypothetical protein -
  AB6M99_RS10260 (AB6M99_10260) - 2083332..2083682 (-) 351 WP_121835625.1 phage head-tail connector protein -
  AB6M99_RS10265 (AB6M99_10265) - 2083697..2083963 (-) 267 WP_121835627.1 hypothetical protein -
  AB6M99_RS10270 (AB6M99_10270) - 2083974..2085020 (-) 1047 WP_369351235.1 major capsid protein -
  AB6M99_RS10275 (AB6M99_10275) - 2085024..2085389 (-) 366 WP_121835631.1 hypothetical protein -
  AB6M99_RS10280 (AB6M99_10280) - 2085407..2086021 (-) 615 WP_121835633.1 DUF4355 domain-containing protein -
  AB6M99_RS10285 (AB6M99_10285) - 2086250..2086456 (-) 207 WP_121835635.1 hypothetical protein -
  AB6M99_RS10290 (AB6M99_10290) - 2086488..2086658 (-) 171 WP_183121664.1 hypothetical protein -
  AB6M99_RS10295 (AB6M99_10295) - 2086655..2086897 (-) 243 WP_121835636.1 hypothetical protein -
  AB6M99_RS10300 (AB6M99_10300) - 2086909..2087184 (-) 276 WP_183121665.1 hypothetical protein -
  AB6M99_RS10305 (AB6M99_10305) - 2087567..2089003 (-) 1437 WP_121835638.1 minor capsid protein -
  AB6M99_RS10310 (AB6M99_10310) - 2088984..2090519 (-) 1536 WP_121835640.1 phage portal protein -
  AB6M99_RS10315 (AB6M99_10315) - 2090530..2091921 (-) 1392 WP_121835642.1 terminase family protein -
  AB6M99_RS10320 (AB6M99_10320) - 2091918..2092370 (-) 453 WP_369351270.1 terminase small subunit -
  AB6M99_RS10325 (AB6M99_10325) - 2092301..2092489 (-) 189 WP_369350729.1 hypothetical protein -
  AB6M99_RS10330 (AB6M99_10330) - 2092693..2093121 (-) 429 WP_121835646.1 DUF1492 domain-containing protein -
  AB6M99_RS10335 (AB6M99_10335) - 2093429..2093653 (-) 225 WP_121835648.1 hypothetical protein -
  AB6M99_RS10340 (AB6M99_10340) - 2093656..2094069 (-) 414 WP_121835650.1 YopX family protein -
  AB6M99_RS10345 (AB6M99_10345) - 2094053..2094307 (-) 255 WP_121835652.1 hypothetical protein -
  AB6M99_RS10350 (AB6M99_10350) - 2094307..2094786 (-) 480 WP_121835653.1 DUF1642 domain-containing protein -
  AB6M99_RS10355 (AB6M99_10355) - 2094788..2094961 (-) 174 WP_183121666.1 hypothetical protein -
  AB6M99_RS10360 (AB6M99_10360) - 2094995..2095234 (-) 240 WP_121835655.1 hypothetical protein -
  AB6M99_RS10365 (AB6M99_10365) - 2095238..2095492 (-) 255 WP_121835657.1 hypothetical protein -
  AB6M99_RS10370 (AB6M99_10370) - 2095494..2095796 (-) 303 WP_121835659.1 DUF1372 family protein -
  AB6M99_RS10375 (AB6M99_10375) - 2095784..2096212 (-) 429 WP_121835661.1 RusA family crossover junction endodeoxyribonuclease -
  AB6M99_RS10380 (AB6M99_10380) - 2096209..2096538 (-) 330 WP_121835662.1 hypothetical protein -
  AB6M99_RS10385 (AB6M99_10385) - 2096551..2097939 (-) 1389 WP_121835664.1 PcfJ domain-containing protein -
  AB6M99_RS10390 (AB6M99_10390) - 2097929..2098399 (-) 471 WP_121835666.1 Cas9 inhibitor AcrIIA9 family protein -
  AB6M99_RS10395 (AB6M99_10395) - 2098644..2099291 (-) 648 WP_121835668.1 hypothetical protein -
  AB6M99_RS10400 (AB6M99_10400) - 2099291..2099608 (-) 318 WP_121835670.1 hypothetical protein -
  AB6M99_RS10405 (AB6M99_10405) - 2099611..2100639 (-) 1029 WP_121835672.1 DUF1351 domain-containing protein -
  AB6M99_RS10410 (AB6M99_10410) bet 2100649..2101497 (-) 849 WP_369350738.1 phage recombination protein Bet -
  AB6M99_RS10415 (AB6M99_10415) - 2101494..2101673 (-) 180 WP_000373551.1 hypothetical protein -
  AB6M99_RS10420 (AB6M99_10420) - 2101677..2101799 (-) 123 WP_281272441.1 hypothetical protein -
  AB6M99_RS10425 (AB6M99_10425) - 2101810..2102070 (-) 261 WP_121835675.1 hypothetical protein -
  AB6M99_RS10430 (AB6M99_10430) - 2102070..2102333 (-) 264 WP_121835677.1 hypothetical protein -
  AB6M99_RS10435 (AB6M99_10435) - 2102530..2102856 (-) 327 WP_245962702.1 HTH domain-containing protein -
  AB6M99_RS10440 (AB6M99_10440) - 2102918..2103094 (-) 177 WP_183121667.1 hypothetical protein -
  AB6M99_RS10445 (AB6M99_10445) - 2103169..2103894 (-) 726 WP_121835681.1 phage antirepressor KilAC domain-containing protein -
  AB6M99_RS10450 (AB6M99_10450) - 2103920..2104126 (-) 207 WP_121835684.1 helix-turn-helix transcriptional regulator -
  AB6M99_RS10455 (AB6M99_10455) - 2104300..2105055 (+) 756 WP_121835686.1 XRE family transcriptional regulator -
  AB6M99_RS10460 (AB6M99_10460) - 2105068..2105880 (+) 813 WP_121835688.1 hypothetical protein -
  AB6M99_RS10465 (AB6M99_10465) - 2106010..2107455 (+) 1446 WP_121835690.1 recombinase family protein -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 17188.83 Da        Isoelectric Point: 6.9520

>NTDB_id=1030323 AB6M99_RS10170 WP_121835590.1 2067268..2067723(-) (comYF) [Streptococcus hillyeri strain S23-3001-2]
MEKRLFVLRNIKLPAFTLLECLVSLLVIIGSVQVYQGLTQVLSSQVHYLSDNRQEDWLLFCQQLQAEFEGTKLIKLENNK
LYLFKGGQKLAFGLSQKGDFRKTNADGRGYQPMIFGIESSAMSQNGNLVTIDMTFTDGLERSFVYAFEKES

Nucleotide


Download         Length: 456 bp        

>NTDB_id=1030323 AB6M99_RS10170 WP_121835590.1 2067268..2067723(-) (comYF) [Streptococcus hillyeri strain S23-3001-2]
ATGGAAAAGAGGTTATTTGTCTTGAGAAACATTAAGTTACCTGCTTTTACGCTCCTAGAATGCTTGGTTTCCTTATTAGT
CATTATTGGGTCTGTGCAAGTTTATCAAGGCTTGACGCAGGTGTTGTCTAGTCAGGTGCATTATTTGTCAGATAATCGAC
AAGAAGATTGGTTGCTCTTTTGCCAGCAATTACAGGCAGAATTTGAAGGGACAAAACTTATCAAACTTGAAAACAACAAA
CTCTACCTATTTAAAGGAGGGCAAAAATTAGCATTCGGGCTATCTCAAAAAGGGGATTTCAGGAAAACCAATGCAGATGG
TCGAGGGTATCAGCCCATGATTTTTGGCATTGAAAGCTCTGCTATGTCCCAAAATGGTAATCTGGTAACGATTGATATGA
CATTCACAGATGGCTTAGAGAGGAGTTTTGTCTATGCTTTTGAAAAAGAAAGTTAG

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(43-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA159

60.714

92.715

0.563
  comYF Streptococcus mutans UA140

60.714

92.715

0.563
  comGF/cglF Streptococcus pneumoniae Rx1

44.366

94.04

0.417
  comGF/cglF Streptococcus pneumoniae D39

44.366

94.04

0.417
  comGF/cglF Streptococcus pneumoniae R6

44.366

94.04

0.417
  comGF/cglF Streptococcus pneumoniae TIGR4

44.366

94.04

0.417
  comGF/cglF Streptococcus mitis NCTC 12261

42.553

93.377

0.397
  comGF/cglF Streptococcus mitis SK321

42.553

93.377

0.397
  comGF Lactococcus lactis subsp. cremoris KW2

43.066

90.728

0.391

Multiple sequence alignment