Detailed information NTG1029867

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AB5991_RS13245
• Genome accession: NZ_CP163447
• Coordinates: 2458366..2458983 (-)
• Length: 205 a.a.
• NCBI ID: WP_014480312.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SD2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2453366..2463983

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB5991_RS13215 (AB5991_13215)	rpsT	2453668..2453934 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
AB5991_RS13220 (AB5991_13220)	holA	2453949..2454992 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
AB5991_RS13225 (AB5991_13225)	-	2455032..2455181 (-)	150	WP_003229985.1	hypothetical protein	-
AB5991_RS13230 (AB5991_13230)	yqzM	2455222..2455356 (+)	135	WP_003229983.1	YqzM family protein	-
AB5991_RS13235 (AB5991_13235)	comEC	2455396..2457726 (-)	2331	WP_033881047.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AB5991_RS13240 (AB5991_13240)	comEB	2457730..2458299 (-)	570	WP_003229978.1	ComE operon protein 2	-
AB5991_RS13245 (AB5991_13245)	comEA	2458366..2458983 (-)	618	WP_014480312.1	competence protein ComEA	Machinery gene
AB5991_RS13250 (AB5991_13250)	comER	2459067..2459888 (+)	822	WP_014480313.1	late competence protein ComER	-
AB5991_RS13255 (AB5991_13255)	yqeM	2459954..2460697 (-)	744	WP_014480314.1	class I SAM-dependent methyltransferase	-
AB5991_RS13260 (AB5991_13260)	rsfS	2460694..2461050 (-)	357	WP_014480315.1	ribosome silencing factor	-
AB5991_RS13265 (AB5991_13265)	yqeK	2461068..2461628 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AB5991_RS13270 (AB5991_13270)	nadD	2461618..2462187 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
AB5991_RS13275 (AB5991_13275)	yhbY	2462199..2462489 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
AB5991_RS13280 (AB5991_13280)	aroE	2462483..2463325 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21781.51 Da
• Isoelectric Point: 4.7220

>NTDB_id=1029867 AB5991_RS13245 WP_014480312.1 2458366..2458983(-) (comEA) [Bacillus subtilis strain SD2]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=1029867 AB5991_RS13245 WP_014480312.1 2458366..2458983(-) (comEA) [Bacillus subtilis strain SD2]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	99.512	100	0.995
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39