Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PHAGEPCYII10_RS27230 Genome accession   NZ_LT608330
Coordinates   5953664..5954287 (-) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa isolate PA14Or_reads     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5948664..5959287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PHAGEPCYII10_RS27210 aroB 5949271..5950377 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  PHAGEPCYII10_RS27215 aroK 5950427..5950945 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PHAGEPCYII10_RS27220 pilQ 5950957..5953089 (-) 2133 WP_003141780.1 type 4a pilus secretin PilQ Machinery gene
  PHAGEPCYII10_RS27225 comL/pilP 5953143..5953667 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PHAGEPCYII10_RS27230 pilO 5953664..5954287 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  PHAGEPCYII10_RS27235 pilN 5954284..5954880 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  PHAGEPCYII10_RS27240 pilM 5954880..5955944 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PHAGEPCYII10_RS27245 - 5956129..5958600 (+) 2472 WP_003141786.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=1029370 PHAGEPCYII10_RS27230 WP_003116135.1 5953664..5954287(-) (pilO) [Pseudomonas aeruginosa isolate PA14Or_reads]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=1029370 PHAGEPCYII10_RS27230 WP_003116135.1 5953664..5954287(-) (pilO) [Pseudomonas aeruginosa isolate PA14Or_reads]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396