Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH75_RS13440 Genome accession   NZ_AP027933
Coordinates   2949097..2950236 (+) Length   379 a.a.
NCBI ID   WP_005361600.1    Uniprot ID   -
Organism   Aeromonas veronii strain VA7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2944097..2955236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH75_RS13410 (VAWG002_26840) - 2944328..2945392 (+) 1065 WP_005353109.1 3-deoxy-7-phosphoheptulonate synthase -
  AACH75_RS13415 (VAWG002_26850) aac(6') 2945399..2945857 (+) 459 WP_338771784.1 aminoglycoside 6'-N-acetyltransferase -
  AACH75_RS13420 (VAWG002_26860) - 2945880..2946677 (-) 798 WP_338775004.1 ATP-binding cassette domain-containing protein -
  AACH75_RS13425 (VAWG002_26870) btuC 2946731..2947738 (-) 1008 WP_338771785.1 vitamin B12 ABC transporter permease BtuC -
  AACH75_RS13430 (VAWG002_26880) - 2947838..2948569 (-) 732 WP_338771786.1 transporter substrate-binding domain-containing protein -
  AACH75_RS13435 (VAWG002_26890) - 2948682..2949038 (+) 357 WP_005337656.1 DMT family protein -
  AACH75_RS13440 (VAWG002_26900) pilU 2949097..2950236 (+) 1140 WP_005361600.1 type IVa pilus ATPase TapW Machinery gene
  AACH75_RS13445 (VAWG002_26910) - 2950331..2950780 (-) 450 WP_338771789.1 isoprenylcysteine carboxylmethyltransferase family protein -
  AACH75_RS13450 (VAWG002_26920) alr 2950954..2952180 (+) 1227 WP_101530485.1 alanine racemase -
  AACH75_RS13455 (VAWG002_26930) - 2952246..2953190 (-) 945 WP_005337664.1 TerC family protein -
  AACH75_RS13460 (VAWG002_26940) oppF 2953493..2954488 (-) 996 WP_005337666.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42239.31 Da        Isoelectric Point: 6.6009

>NTDB_id=102918 AACH75_RS13440 WP_005361600.1 2949097..2950236(+) (pilU) [Aeromonas veronii strain VA7]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKQQFEKDLEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTDIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKGDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=102918 AACH75_RS13440 WP_005361600.1 2949097..2950236(+) (pilU) [Aeromonas veronii strain VA7]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAATTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAGCCGGGGGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGAAGGATCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTTTGGTGGCCCGCAACATCAAGACCGACATCCCCCGCTTTGA
AGATCTCAAACTCCCGCCTGTGCTGCTCGACACCATCATGGAGAAACGCGGTCTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGTAAATCTACCTCGCTGGCGGCGCTTATCGACCACCGCAACCGCAACAGCGGCGGGCACATCATTACCATCGAGGAT
CCGGTGGAGTTCGTTCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTTGGCGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATTCTTATCGGCGAAATTCGCGATCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCCCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCTTTTGTCTCCCAGCG
GCTGGTGAAAACCACTGATGGCGGCCGCCGGGCCGCAGTGGAGATCATGCTCGGCACCCACACCATCCGCGACATGATCA
AACGCGGCGAATTTGGTGGTCTCAAAGAGGTGATGGAAAAATCGAAGGCCCTCGGCATGGTTACCTTCGACAGTGCCCTG
TTCGACCTGGTGGTGGAAGGGGTGATTGAGGAAGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGGCAGATTGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGGCGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.474

100

0.546
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Pseudomonas aeruginosa PAK

41.279

90.765

0.375
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

87.863

0.367
  pilT Vibrio cholerae strain A1552

41.742

87.863

0.367
  pilT Acinetobacter baumannii D1279779

41.617

88.127

0.367
  pilT Acinetobacter baumannii strain A118

41.617

88.127

0.367
  pilT Acinetobacter nosocomialis M2

41.617

88.127

0.367

Multiple sequence alignment