Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH72_RS10825 Genome accession   NZ_AP027931
Coordinates   2414030..2415139 (+) Length   369 a.a.
NCBI ID   WP_010636179.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain VA6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2409030..2420139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH72_RS10790 (VAWG001_21130) - 2409638..2409949 (+) 312 WP_005304992.1 hypothetical protein -
  AACH72_RS10795 (VAWG001_21140) - 2410012..2410431 (-) 420 WP_005304995.1 DUF4426 domain-containing protein -
  AACH72_RS10800 (VAWG001_21150) yggU 2410450..2410749 (-) 300 WP_005304998.1 DUF167 family protein YggU -
  AACH72_RS10805 (VAWG001_21160) - 2410749..2411300 (-) 552 WP_017780458.1 YggT family protein -
  AACH72_RS10810 (VAWG001_21170) proC 2411341..2412165 (-) 825 WP_017780457.1 pyrroline-5-carboxylate reductase -
  AACH72_RS10815 (VAWG001_21180) - 2412226..2412927 (-) 702 WP_213344242.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH72_RS10820 (VAWG001_21190) pilT 2412967..2414001 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  AACH72_RS10825 (VAWG001_21200) pilU 2414030..2415139 (+) 1110 WP_010636179.1 type IVa pilus ATPase TapU Machinery gene
  AACH72_RS10830 (VAWG001_21210) yaaA 2415171..2415944 (+) 774 WP_017783834.1 peroxide stress protein YaaA -
  AACH72_RS10835 (VAWG001_21220) srmB 2416121..2417344 (-) 1224 WP_010636177.1 ATP-dependent RNA helicase SrmB -
  AACH72_RS10840 (VAWG001_21230) - 2417473..2418183 (+) 711 WP_124249317.1 methyltransferase -
  AACH72_RS10845 (VAWG001_21240) brnQ 2418374..2419681 (+) 1308 WP_005305030.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41081.22 Da        Isoelectric Point: 6.5672

>NTDB_id=102884 AACH72_RS10825 WP_010636179.1 2414030..2415139(+) (pilU) [Aeromonas dhakensis strain VA6]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=102884 AACH72_RS10825 WP_010636179.1 2414030..2415139(+) (pilU) [Aeromonas dhakensis strain VA6]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAATGGCCACCTGCAGTCGCTGGCAGAAGCGCCCCTCGACAAGGCAAGGGCACTGGCGCTGGTGAAGGAGA
CCCTGAGCCCGGAGCACTTCGAACGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCACTGCCCCCCATATTGCAGGAGATCGCCCTCGCCAAGCGCGGGTTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGAAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAGAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTACAATTTGCCGAGACCGGTCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCACCTGGTGCCCCAGGAGAAACACCGTCAGTTCCTGTTCGATCTCTCTTTCAATCTGCGCGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGACAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAACAGCTGGGTTCAGGCACACTCGACAACGTTACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.87

99.729

0.607
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363

Multiple sequence alignment