Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DQN52_RS10545 Genome accession   NZ_LS483426
Coordinates   1953402..1954682 (-) Length   426 a.a.
NCBI ID   WP_003787551.1    Uniprot ID   F5S877
Organism   Kingella kingae strain NCTC10529     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1948402..1959682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN52_RS10520 (NCTC10529_02049) folD 1949423..1950274 (+) 852 WP_003787562.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD -
  DQN52_RS10525 (NCTC10529_02050) - 1950417..1951802 (+) 1386 WP_003787560.1 GntP family permease -
  DQN52_RS10530 (NCTC10529_02051) - 1951927..1952436 (+) 510 WP_003787558.1 gluconokinase -
  DQN52_RS10535 (NCTC10529_02052) - 1952507..1952884 (-) 378 WP_003787555.1 diacylglycerol kinase -
  DQN52_RS10540 (NCTC10529_02053) - 1952881..1953324 (-) 444 WP_003787553.1 peroxiredoxin -
  DQN52_RS10545 (NCTC10529_02054) pilU 1953402..1954682 (-) 1281 WP_003787551.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DQN52_RS10550 (NCTC10529_02055) pilT 1954702..1955742 (-) 1041 WP_003791105.1 type IV pilus twitching motility protein PilT Machinery gene
  DQN52_RS10555 (NCTC10529_02056) pseC 1955948..1957105 (-) 1158 WP_003787545.1 UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine transaminase -
  DQN52_RS10560 (NCTC10529_02057) - 1957218..1958393 (-) 1176 WP_003787543.1 alpha-hydroxy acid oxidase -

Sequence

Protein


Download         Length: 426 a.a.        Molecular weight: 47321.06 Da        Isoelectric Point: 6.2389

>NTDB_id=1024407 DQN52_RS10545 WP_003787551.1 1953402..1954682(-) (pilU) [Kingella kingae strain NCTC10529]
MAEQDITSLLEGFVNNYKVTNDAAKPAAPAGRAVPASAQATATASEIAPAKKSGLHPLLEKMAAECEKRGASDIFISAGF
PPSFKINGELTPVPLKPLTQQDTQILVYSTMSPDQRARFDHELELNYSVSSSEGIRFRVNSYHEQGRMGMVWRRITTKIL
TVDDLQLPQGLKDLAMQPRGLLILAGATGSGKSTSMAAMLDWRNKHAAGHIVTIEDPVEYLHKPIKSIFTQRELGVDTNS
WSMAVQSAMRQAPDVVCVGEVRNEHTMEYALQLAQTGHLCIFTIHANNAVQTIERIMNFYPEERHQQVLMDLALNTVGII
GQRLARLKGGGRTAIVDLLINSPAMQDHIFKGELMEAMSLMNRSETDGMQTFDQDLFRLYTEDKITYEEALRQAESVNDL
RLKIKLWEEGNAKDHIFERVGGLSLQ

Nucleotide


Download         Length: 1281 bp        

>NTDB_id=1024407 DQN52_RS10545 WP_003787551.1 1953402..1954682(-) (pilU) [Kingella kingae strain NCTC10529]
ATGGCAGAACAAGACATCACTTCCCTACTTGAAGGTTTTGTAAACAATTACAAAGTAACCAACGATGCCGCCAAGCCTGC
TGCACCAGCAGGACGAGCCGTGCCTGCATCGGCACAAGCCACGGCAACGGCTTCGGAAATTGCGCCAGCCAAAAAATCGG
GCTTACACCCTTTGTTGGAAAAAATGGCTGCCGAATGTGAAAAACGCGGTGCATCGGATATTTTTATCAGCGCAGGTTTT
CCGCCATCATTCAAAATCAATGGCGAATTAACGCCTGTTCCGCTCAAACCGCTCACGCAGCAAGACACGCAAATTTTGGT
GTATTCCACTATGTCGCCAGACCAACGTGCGCGTTTTGACCACGAATTGGAATTGAACTACTCCGTGTCGTCTAGCGAGG
GCATTCGCTTTCGTGTGAACTCGTATCACGAACAAGGTCGCATGGGCATGGTGTGGCGTCGCATCACCACCAAAATCTTA
ACGGTTGATGATTTGCAGTTGCCACAAGGTTTAAAAGACTTGGCGATGCAGCCGCGCGGTTTGTTGATTTTGGCAGGCGC
AACGGGTTCGGGTAAATCTACGTCTATGGCGGCGATGCTGGACTGGCGTAACAAACACGCCGCAGGTCATATTGTGACCA
TTGAAGACCCTGTGGAATATCTGCACAAACCAATCAAGAGTATTTTCACGCAACGCGAATTGGGCGTGGATACGAATTCT
TGGTCAATGGCGGTGCAATCGGCAATGCGTCAAGCACCAGACGTGGTTTGTGTGGGTGAGGTGCGTAACGAACACACCAT
GGAATACGCTTTGCAACTGGCGCAAACAGGTCACTTGTGTATCTTTACCATTCATGCGAACAATGCCGTGCAAACCATTG
AGCGTATTATGAACTTCTATCCAGAAGAGCGTCATCAACAAGTATTGATGGACTTGGCGTTGAACACAGTGGGCATTATT
GGTCAGCGTTTGGCGCGATTGAAAGGCGGCGGACGTACGGCGATTGTGGATTTGTTGATTAACAGCCCTGCGATGCAAGA
CCACATTTTCAAAGGCGAGTTGATGGAAGCGATGTCGTTGATGAATCGTTCGGAAACAGACGGCATGCAAACGTTTGACC
AAGACTTGTTCCGCTTGTACACCGAAGACAAAATCACTTACGAAGAAGCCTTGCGCCAAGCTGAATCGGTAAACGACTTG
CGCTTGAAAATCAAATTGTGGGAAGAGGGTAATGCCAAAGACCACATCTTTGAGCGCGTGGGCGGTTTGAGCTTGCAATA
A

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(96-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5S877

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

47.696

86.62

0.413
  pilU Acinetobacter baylyi ADP1

49.711

81.221

0.404
  pilU Vibrio cholerae strain A1552

46.429

78.873

0.366