Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AB2S62_RS01305 Genome accession   NZ_CP162100
Coordinates   268345..269340 (+) Length   331 a.a.
NCBI ID   WP_367987936.1    Uniprot ID   -
Organism   Vibrio sp. NTOU-M3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 263345..274340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2S62_RS01290 (AB2S62_01050) - 263885..264613 (-) 729 WP_367987933.1 glutathione peroxidase -
  AB2S62_RS01295 (AB2S62_01055) oxyR 264765..265655 (+) 891 WP_367987934.1 DNA-binding transcriptional regulator OxyR -
  AB2S62_RS01300 (AB2S62_01060) - 265695..268202 (-) 2508 WP_367987935.1 penicillin-binding protein 1A -
  AB2S62_RS01305 (AB2S62_01065) pilM 268345..269340 (+) 996 WP_367987936.1 type IV pilus assembly protein PilM Machinery gene
  AB2S62_RS01310 (AB2S62_01070) pilN 269330..269920 (+) 591 WP_367987937.1 PilN domain-containing protein Machinery gene
  AB2S62_RS01315 (AB2S62_01075) pilO 269913..270503 (+) 591 WP_367987938.1 type 4a pilus biogenesis protein PilO Machinery gene
  AB2S62_RS01320 (AB2S62_01080) pilP 270493..271011 (+) 519 WP_367987939.1 pilus assembly protein PilP Machinery gene
  AB2S62_RS01325 (AB2S62_01085) pilQ 271097..272767 (+) 1671 WP_374144809.1 type IV pilus secretin PilQ Machinery gene
  AB2S62_RS01330 (AB2S62_01090) aroK 272943..273461 (+) 519 WP_367987941.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 37176.88 Da        Isoelectric Point: 7.0148

>NTDB_id=1024023 AB2S62_RS01305 WP_367987936.1 268345..269340(+) (pilM) [Vibrio sp. NTOU-M3]
MGKSLVTGIDIGHHSIKAVVLKPVKGTFTLAGYKELPIEADIFSDNHMLNYQKIVKKLKALRKGLPLFSSRVALSVPDNT
VISKVLQIDSELDDREREFAIFQNFSHQSPFPIEELHLDYIELPKKTVSQTTVSYQVYATKKDLVESRVNAAEKAGFQPF
LMEMQAHSLTHVWQKVSQQENKSDWLLVDIGLSQTLMCIDFADKAPFHKELALGTQVIDAKGEYEHLMHSSTERFIFGLI
EKLQRNIQILGSVHGTPIKGIWLTGGGAATPMLVEEIQRRLEVECEVLNPLHLFKGKRSKHTAEMENGFAFSTAVGLALR
GIDWMEQSHAA

Nucleotide


Download         Length: 996 bp        

>NTDB_id=1024023 AB2S62_RS01305 WP_367987936.1 268345..269340(+) (pilM) [Vibrio sp. NTOU-M3]
ATGGGTAAATCACTAGTCACTGGCATTGACATTGGTCACCACAGTATAAAAGCAGTGGTGTTAAAGCCAGTTAAAGGCAC
ATTCACACTTGCTGGTTACAAAGAGTTGCCAATTGAAGCAGACATTTTCTCTGATAACCACATGTTAAATTATCAGAAAA
TTGTAAAGAAACTCAAAGCGCTAAGGAAGGGTTTACCATTATTTAGCAGCAGAGTCGCGCTTTCGGTACCCGACAACACC
GTAATAAGCAAAGTATTACAAATAGATAGTGAGCTCGATGATAGAGAAAGAGAGTTCGCTATTTTTCAGAATTTTTCTCA
TCAATCCCCATTTCCAATTGAAGAACTGCATTTGGATTACATCGAACTACCGAAAAAAACGGTTAGCCAGACCACGGTAA
GCTATCAAGTTTATGCCACAAAGAAAGATCTGGTGGAAAGTCGAGTTAATGCGGCTGAAAAAGCGGGATTTCAGCCTTTT
TTAATGGAGATGCAAGCACACAGCCTTACTCATGTGTGGCAAAAAGTGTCACAGCAAGAAAATAAGTCAGACTGGTTATT
GGTTGATATTGGATTAAGTCAGACTTTGATGTGCATCGATTTTGCAGATAAAGCCCCTTTTCATAAAGAGCTGGCATTAG
GTACACAAGTGATTGATGCAAAAGGGGAGTATGAGCATCTCATGCACAGCAGTACCGAACGTTTCATTTTTGGCTTAATT
GAAAAACTACAACGCAACATTCAAATCCTTGGCTCTGTCCACGGCACGCCCATTAAAGGCATTTGGCTGACAGGGGGAGG
AGCTGCAACCCCGATGTTAGTTGAAGAGATTCAGCGCCGATTAGAAGTGGAATGCGAAGTATTAAACCCCCTGCACCTAT
TTAAAGGCAAACGCAGTAAACATACGGCAGAAATGGAAAATGGTTTTGCCTTCAGTACCGCTGTTGGGCTGGCATTAAGA
GGCATTGACTGGATGGAGCAATCTCATGCTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(235-321)

Type IV pilus assembly protein PilM; (PilM_2)

(8-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.734

100

0.559
  pilM Vibrio campbellii strain DS40M4

44.179

100

0.447

Multiple sequence alignment