Detailed information NTG1023452

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB3G31_RS07305
• Genome accession: NZ_CP162006
• Coordinates: 1524409..1525665 (-)
• Length: 418 a.a.
• NCBI ID: WP_367849527.1
• Uniprot ID: -
• Organism: Rhodoferax sp. WC2427
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1519409..1530665

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB3G31_RS07290 (AB3G31_07290)	-	1520729..1522192 (+)	1464	WP_367849524.1	DEAD/DEAH box helicase	-
AB3G31_RS07295 (AB3G31_07295)	-	1522245..1523090 (-)	846	WP_367849525.1	symmetrical bis(5'-nucleosyl)-tetraphosphatase	-
AB3G31_RS07300 (AB3G31_07300)	-	1523114..1524412 (+)	1299	WP_367849526.1	hemolysin family protein	-
AB3G31_RS07305 (AB3G31_07305)	pilU	1524409..1525665 (-)	1257	WP_367849527.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB3G31_RS07310 (AB3G31_07310)	purM	1525744..1526784 (-)	1041	WP_367849528.1	phosphoribosylformylglycinamidine cyclo-ligase	-
AB3G31_RS07315 (AB3G31_07315)	-	1526972..1528042 (+)	1071	WP_367849529.1	AI-2E family transporter	-
AB3G31_RS07320 (AB3G31_07320)	hda	1528049..1528720 (+)	672	WP_367849530.1	DnaA regulatory inactivator Hda	-
AB3G31_RS07325 (AB3G31_07325)	-	1528732..1529406 (+)	675	WP_367849531.1	HAD family hydrolase	-

Sequence

Protein

• Length: 418 a.a.
• Molecular weight: 46370.11 Da
• Isoelectric Point: 7.2164

>NTDB_id=1023452 AB3G31_RS07305 WP_367849527.1 1524409..1525665(-) (pilU) [Rhodoferax sp. WC2427]
MSALTEDQARSYMHKLLAAMHQAGGSDLFIASDFAPSMKAHGEMKPLSAQKLSGDVTRMLAQALMNERQNAEFAKEMECN
FAMALPGVARFRVNVFVQQQNVSMVIRTIAAEIPSFATLALPPVLKEVIMHKRGLVLVVGGTGSGKSTSLAAMIDHRNSS
SGGHIITVEDPVEYVHTSKMSLVSHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDPQTMEHAIAFAETGHLCLATLHA
NSANQALDRIINFFPEERRNQLLMDLSANLRAIVSQRLVRTEDGKGRRAAVEILLNTPLVADRIFKGQFGDLKALMEKSR
ELGMRTFDWALFELYNDGHISYEEALRNADSTNELRLNMKLKSARGEPASAGGTELTFDKALSAEELEALRAEELLKQQQ
KRRQLEDEQLARLRQAKG

Nucleotide

• Length: 1257 bp

>NTDB_id=1023452 AB3G31_RS07305 WP_367849527.1 1524409..1525665(-) (pilU) [Rhodoferax sp. WC2427]
ATGTCCGCACTCACCGAAGACCAGGCCCGCAGTTACATGCACAAGCTGCTGGCGGCGATGCACCAGGCGGGTGGCTCGGA
CCTGTTCATCGCCAGCGACTTCGCGCCCAGCATGAAGGCGCACGGCGAGATGAAACCGCTGTCGGCCCAAAAGCTCAGCG
GCGACGTGACCCGCATGCTGGCCCAGGCGCTGATGAATGAGCGGCAAAACGCCGAGTTCGCCAAGGAAATGGAGTGCAAC
TTCGCCATGGCGCTGCCGGGCGTGGCGCGGTTCCGGGTGAATGTGTTTGTGCAGCAGCAAAACGTCAGCATGGTGATCCG
CACCATCGCGGCCGAGATCCCCAGCTTCGCCACCCTGGCGCTGCCGCCGGTGCTGAAGGAAGTCATCATGCACAAGCGCG
GGCTGGTGCTGGTGGTGGGCGGCACGGGGTCGGGCAAATCGACCTCGCTGGCGGCCATGATCGACCACCGCAACAGCAGC
TCGGGCGGCCACATCATCACCGTAGAAGACCCGGTGGAGTACGTGCACACCTCGAAGATGTCGCTGGTGAGCCACCGCGA
GGTGGGGGTGGACACGCATTCCTGGCACCACGCGCTGAAAAACACCCTGCGCCAGGCCCCGGACGTGATTCTGATCGGCG
AAATCCGCGATCCGCAGACCATGGAGCATGCGATCGCCTTTGCCGAAACCGGCCACCTGTGCCTGGCCACACTGCACGCC
AACAGCGCCAACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGGCGCAACCAGCTGCTGATGGATTTGTC
GGCCAATCTGCGGGCCATCGTGTCGCAGCGGCTGGTGCGCACCGAAGACGGCAAGGGCCGCCGCGCCGCGGTCGAGATTT
TGCTCAATACGCCGCTGGTGGCGGACCGGATCTTCAAGGGCCAGTTTGGCGACCTCAAGGCGCTGATGGAGAAGTCGCGC
GAGCTGGGCATGCGCACCTTCGACTGGGCCCTGTTCGAGCTGTACAACGACGGCCACATCTCCTACGAGGAGGCGCTGCG
CAACGCCGACTCCACCAACGAGCTGCGCCTGAACATGAAGCTCAAGAGCGCCCGGGGCGAGCCCGCCAGCGCGGGCGGTA
CGGAGCTGACCTTTGACAAAGCGCTGTCAGCCGAAGAGCTGGAAGCCCTGCGCGCCGAAGAACTGCTCAAACAGCAGCAA
AAACGCCGCCAGCTGGAAGACGAGCAGTTGGCCCGGCTGCGGCAGGCAAAAGGTTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (59-281)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.026	83.493	0.493
pilU	Acinetobacter baylyi ADP1	53.955	84.689	0.457
pilU	Vibrio cholerae strain A1552	49.722	86.124	0.428