Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB0R90_RS12855 Genome accession   NZ_CP160217
Coordinates   2611504..2612574 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain AP52     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2606504..2617574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R90_RS12805 (AB0R90_12805) sipW 2606564..2607148 (-) 585 WP_012117977.1 signal peptidase I SipW -
  AB0R90_RS12810 (AB0R90_12810) tapA 2607120..2607791 (-) 672 WP_020954230.1 amyloid fiber anchoring/assembly protein TapA -
  AB0R90_RS12815 (AB0R90_12815) - 2608050..2608379 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  AB0R90_RS12820 (AB0R90_12820) - 2608419..2608598 (-) 180 WP_059368116.1 YqzE family protein -
  AB0R90_RS12825 (AB0R90_12825) comGG 2608655..2609032 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  AB0R90_RS12830 (AB0R90_12830) comGF 2609033..2609533 (-) 501 WP_257474763.1 competence type IV pilus minor pilin ComGF -
  AB0R90_RS12835 (AB0R90_12835) comGE 2609442..2609756 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  AB0R90_RS12840 (AB0R90_12840) comGD 2609740..2610177 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  AB0R90_RS12845 (AB0R90_12845) comGC 2610167..2610475 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  AB0R90_RS12850 (AB0R90_12850) comGB 2610480..2611517 (-) 1038 WP_099566903.1 competence type IV pilus assembly protein ComGB Machinery gene
  AB0R90_RS12855 (AB0R90_12855) comGA 2611504..2612574 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  AB0R90_RS12860 (AB0R90_12860) - 2612771..2613721 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  AB0R90_RS12865 (AB0R90_12865) - 2613867..2615168 (+) 1302 WP_012117986.1 hemolysin family protein -
  AB0R90_RS12870 (AB0R90_12870) - 2615206..2616036 (-) 831 WP_015240214.1 STAS domain-containing protein -
  AB0R90_RS12880 (AB0R90_12880) - 2616243..2616623 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AB0R90_RS12885 (AB0R90_12885) - 2616860..2617105 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=1019473 AB0R90_RS12855 WP_012117985.1 2611504..2612574(-) (comGA) [Bacillus velezensis strain AP52]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1019473 AB0R90_RS12855 WP_012117985.1 2611504..2612574(-) (comGA) [Bacillus velezensis strain AP52]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAATTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCGAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment