Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ABXV02_RS09250 Genome accession   NZ_CP159912
Coordinates   1739027..1739644 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain PY79     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1734027..1744644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXV02_RS09215 aroE 1734685..1735527 (+) 843 WP_003229967.1 shikimate dehydrogenase -
  ABXV02_RS09220 yhbY 1735521..1735811 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  ABXV02_RS09225 nadD 1735823..1736392 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  ABXV02_RS09230 yqeK 1736382..1736942 (+) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ABXV02_RS09235 rsfS 1736960..1737316 (+) 357 WP_003229971.1 ribosome silencing factor -
  ABXV02_RS09240 yqeM 1737313..1738056 (+) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  ABXV02_RS09245 comER 1738122..1738943 (-) 822 WP_004398597.1 late competence protein ComER -
  ABXV02_RS09250 comEA 1739027..1739644 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  ABXV02_RS09255 comEB 1739711..1740280 (+) 570 WP_003229978.1 ComE operon protein 2 -
  ABXV02_RS09260 comEC 1740284..1742614 (+) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ABXV02_RS09265 yqzM 1742654..1742788 (-) 135 WP_003229983.1 YqzM family protein -
  ABXV02_RS09270 - 1742829..1742978 (+) 150 WP_003229985.1 hypothetical protein -
  ABXV02_RS09275 holA 1743018..1744061 (+) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  ABXV02_RS09280 rpsT 1744076..1744342 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=1016736 ABXV02_RS09250 WP_004398514.1 1739027..1739644(+) (comEA) [Bacillus subtilis strain PY79]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=1016736 ABXV02_RS09250 WP_004398514.1 1739027..1739644(+) (comEA) [Bacillus subtilis strain PY79]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment