Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   JMW95_RS03385 Genome accession   NZ_LR890651
Coordinates   701071..701730 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain MINF_2E isolate MINF_2E     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 696071..706730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMW95_RS03360 parE 696162..698054 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  JMW95_RS03365 ygiN 698102..698416 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  JMW95_RS03370 mdaB 698447..699028 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  JMW95_RS03375 ygiZ 699347..699679 (+) 333 WP_000912120.1 DUF2645 family protein -
  JMW95_RS03380 qseC 699725..701074 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  JMW95_RS03385 ciaR 701071..701730 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  JMW95_RS03390 ygiW 701882..702274 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  JMW95_RS03395 ygiV 702327..702809 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  JMW95_RS03400 parC 703355..705613 (+) 2259 WP_001281841.1 DNA topoisomerase IV subunit A -
  JMW95_RS03405 plsC 705847..706584 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=1014863 JMW95_RS03385 WP_001221502.1 701071..701730(-) (ciaR) [Escherichia coli strain MINF_2E isolate MINF_2E]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=1014863 JMW95_RS03385 WP_001221502.1 701071..701730(-) (ciaR) [Escherichia coli strain MINF_2E isolate MINF_2E]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAAGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment