Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   JMW03_RS03805 Genome accession   NZ_LR890349
Coordinates   791688..792347 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain MSB1_9D isolate MSB1_9D     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 786688..797347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMW03_RS03780 parE 786779..788671 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  JMW03_RS03785 ygiN 788719..789033 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  JMW03_RS03790 mdaB 789064..789645 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  JMW03_RS03795 ygiZ 789964..790296 (+) 333 WP_000917684.1 DUF2645 family protein -
  JMW03_RS03800 qseC 790342..791691 (-) 1350 WP_201514321.1 quorum sensing histidine kinase QseC -
  JMW03_RS03805 ciaR 791688..792347 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  JMW03_RS03810 ygiW 792499..792891 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  JMW03_RS03815 ygiV 792944..793426 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  JMW03_RS03820 mqsR 793631..793927 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  JMW03_RS03825 mqsA 793929..794324 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  JMW03_RS03830 ygiS 794457..796064 (+) 1608 WP_201514323.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=1014095 JMW03_RS03805 WP_001221493.1 791688..792347(-) (ciaR) [Escherichia coli strain MSB1_9D isolate MSB1_9D]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=1014095 JMW03_RS03805 WP_001221493.1 791688..792347(-) (ciaR) [Escherichia coli strain MSB1_9D isolate MSB1_9D]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment