Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ABK447_RS07635 Genome accession   NZ_CP157943
Coordinates   1467603..1468673 (+) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus sp. hwrm1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1462603..1473673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABK447_RS07605 (ABK447_07595) - 1463074..1463319 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  ABK447_RS07610 (ABK447_07600) - 1463557..1463937 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ABK447_RS07620 (ABK447_07610) - 1464144..1464974 (+) 831 WP_007408317.1 STAS domain-containing protein -
  ABK447_RS07625 (ABK447_07615) - 1465012..1466313 (-) 1302 WP_021494315.1 hemolysin family protein -
  ABK447_RS07630 (ABK447_07620) - 1466459..1467409 (+) 951 WP_007408319.1 magnesium transporter CorA family protein -
  ABK447_RS07635 (ABK447_07625) comGA 1467603..1468673 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  ABK447_RS07640 (ABK447_07630) comGB 1468660..1469697 (+) 1038 WP_094031834.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABK447_RS07645 (ABK447_07635) comGC 1469744..1470010 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  ABK447_RS07650 (ABK447_07640) comGD 1470000..1470437 (+) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABK447_RS07655 (ABK447_07645) comGE 1470421..1470735 (+) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  ABK447_RS07660 (ABK447_07650) comGF 1470644..1471144 (+) 501 WP_257899725.1 competence type IV pilus minor pilin ComGF -
  ABK447_RS07665 (ABK447_07655) comGG 1471145..1471522 (+) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABK447_RS07670 (ABK447_07660) - 1471579..1471758 (+) 180 WP_003153093.1 YqzE family protein -
  ABK447_RS07675 (ABK447_07665) - 1471798..1472127 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  ABK447_RS07680 (ABK447_07670) tapA 1472386..1473057 (+) 672 WP_124934997.1 amyloid fiber anchoring/assembly protein TapA -
  ABK447_RS07685 (ABK447_07675) sipW 1473029..1473613 (+) 585 WP_015240205.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=1009180 ABK447_RS07635 WP_003153083.1 1467603..1468673(+) (comGA) [Bacillus sp. hwrm1]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1009180 ABK447_RS07635 WP_003153083.1 1467603..1468673(+) (comGA) [Bacillus sp. hwrm1]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment