Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ABLU28_RS10150 Genome accession   NZ_CP157495
Coordinates   2024779..2026101 (+) Length   440 a.a.
NCBI ID   WP_058221332.1    Uniprot ID   -
Organism   Lactococcus lactis strain 2B-1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2019779..2031101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLU28_RS10125 (ABLU28_10095) glyQ 2020295..2021248 (-) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -
  ABLU28_RS10130 (ABLU28_10100) - 2021533..2022279 (-) 747 WP_003130666.1 bifunctional metallophosphatase/5'-nucleotidase -
  ABLU28_RS10135 (ABLU28_10105) - 2022395..2023414 (-) 1020 WP_012897709.1 DUF475 domain-containing protein -
  ABLU28_RS10140 (ABLU28_10110) - 2023462..2023959 (-) 498 WP_003130662.1 VanZ family protein -
  ABLU28_RS10145 (ABLU28_10115) - 2024093..2024722 (-) 630 WP_003130660.1 YigZ family protein -
  ABLU28_RS10150 (ABLU28_10120) comFA 2024779..2026101 (+) 1323 WP_058221332.1 DEAD/DEAH box helicase Machinery gene
  ABLU28_RS10155 (ABLU28_10125) comFC 2026344..2026748 (+) 405 WP_003130657.1 ComF family protein Machinery gene
  ABLU28_RS10160 (ABLU28_10130) - 2026781..2027359 (-) 579 WP_003130655.1 nucleotidyltransferase family protein -
  ABLU28_RS10165 (ABLU28_10135) - 2027373..2027828 (-) 456 WP_003130653.1 diacylglycerol kinase family protein -
  ABLU28_RS10170 (ABLU28_10140) ybeY 2027812..2028300 (-) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  ABLU28_RS10175 (ABLU28_10145) - 2028452..2028931 (-) 480 WP_003130650.1 NUDIX hydrolase -
  ABLU28_RS10180 (ABLU28_10150) - 2028989..2029957 (-) 969 Protein_1957 PhoH family protein -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50201.71 Da        Isoelectric Point: 10.1065

>NTDB_id=1007205 ABLU28_RS10150 WP_058221332.1 2024779..2026101(+) (comFA) [Lactococcus lactis strain 2B-1]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=1007205 ABLU28_RS10150 WP_058221332.1 2024779..2026101(+) (comFA) [Lactococcus lactis strain 2B-1]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-233)

Helicase conserved C-terminal domain (Helicase_C)

(300-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.5

90.909

0.677
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

38.018

98.636

0.375

Multiple sequence alignment