Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ABGM96_RS16125 Genome accession   NZ_CP157360
Coordinates   3361387..3362472 (-) Length   361 a.a.
NCBI ID   WP_373689754.1    Uniprot ID   -
Organism   Peribacillus frigoritolerans strain C1141     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3356387..3367472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABGM96_RS16085 - 3357279..3358076 (+) 798 WP_048688306.1 YqhG family protein -
  ABGM96_RS16090 - 3358121..3358306 (-) 186 WP_048688303.1 YqzE family protein -
  ABGM96_RS16095 comGG 3358399..3358785 (-) 387 WP_048688300.1 competence type IV pilus minor pilin ComGG -
  ABGM96_RS16100 comGF 3358778..3359257 (-) 480 WP_081088666.1 competence type IV pilus minor pilin ComGF -
  ABGM96_RS16105 - 3359205..3359543 (-) 339 WP_048688295.1 hypothetical protein -
  ABGM96_RS16110 comGD 3359530..3359973 (-) 444 WP_048688291.1 competence type IV pilus minor pilin ComGD -
  ABGM96_RS16115 comGC 3359981..3360292 (-) 312 WP_048688337.1 competence type IV pilus major pilin ComGC -
  ABGM96_RS16120 comGB 3360369..3361403 (-) 1035 WP_065421218.1 competence type IV pilus assembly protein ComGB -
  ABGM96_RS16125 comGA 3361387..3362472 (-) 1086 WP_373689754.1 competence type IV pilus ATPase ComGA Machinery gene
  ABGM96_RS16130 - 3362568..3362948 (-) 381 WP_048682187.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ABGM96_RS16135 - 3363164..3363865 (+) 702 WP_048682184.1 helix-turn-helix transcriptional regulator -
  ABGM96_RS16140 - 3363951..3364193 (+) 243 WP_034308393.1 DUF2626 domain-containing protein -
  ABGM96_RS16145 - 3364246..3365346 (-) 1101 WP_072273817.1 class I SAM-dependent methyltransferase -
  ABGM96_RS16150 - 3365654..3366280 (-) 627 WP_048682180.1 MBL fold metallo-hydrolase -
  ABGM96_RS16155 - 3366514..3366687 (+) 174 WP_034308396.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 40134.81 Da        Isoelectric Point: 8.1731

>NTDB_id=1006273 ABGM96_RS16125 WP_373689754.1 3361387..3362472(-) (comGA) [Peribacillus frigoritolerans strain C1141]
MNELISIEKTAEKILTRAIVESASDIHIFFRGEGPLIQFRIDNKLVPQETLSFFEAERLIAHLKFLASMDIGEKRRPQSG
AITINLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSL
LHHAHEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILT
TMHTRDAQGAISRLLEFGVSLLEVEQTLIGVTAQRLVELRCLTCKGDCALACKMTARNKRASVYELLYGKSLAEVLRIMG
DEKGKATVSYRQLKDEIGKAVAMGYVDAQEYERLVYHETKK

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=1006273 ABGM96_RS16125 WP_373689754.1 3361387..3362472(-) (comGA) [Peribacillus frigoritolerans strain C1141]
ATGAATGAATTGATATCGATTGAAAAGACCGCAGAAAAAATACTAACCCGTGCCATCGTGGAGTCGGCATCGGATATCCA
CATTTTTTTTCGCGGAGAGGGGCCTCTCATCCAATTCAGGATAGACAATAAGCTTGTTCCCCAGGAAACATTATCATTTT
TTGAAGCTGAAAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGC
GCCATCACCATCAATTTGGCTAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGT
CATCCGCTTAATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTG
CACTCCTGAAGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGGAAAACCACGACTTTATATTCCCTG
CTTCACCACGCCCATGAGATGATCAATCGAAATATCATTACACTTGAAGACCCCATTGAAAATGTATCCGAAAAGGTATT
GCAAGTCCAAATCAATGAAAAGGCGGGCATTACGTATTCCGTCGGCCTAAAAGCTGTTCTGAGGCATGACCCGGACGTGA
TCATGGTTGGGGAAGTCAGGGATGCAGAAACTGCCAAAATCGCAGTCCGTGCCGCATTAACCGGTCATTTGATACTAACG
ACCATGCATACGAGGGATGCCCAGGGTGCCATCTCCAGATTACTGGAATTTGGTGTCAGCTTACTTGAGGTTGAACAGAC
TTTGATTGGCGTGACAGCGCAGCGGCTGGTTGAATTGCGATGTCTTACGTGTAAAGGGGACTGTGCTTTAGCTTGCAAAA
TGACTGCGAGGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGAAAAAGTCTGGCTGAAGTTCTCCGGATAATGGGA
GATGAAAAAGGAAAGGCAACGGTCAGCTACCGCCAATTAAAGGATGAAATCGGAAAAGCGGTTGCGATGGGCTATGTGGA
TGCTCAGGAATATGAACGGCTGGTATACCATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.056

98.338

0.551
  pilB Haemophilus influenzae 86-028NP

38.506

96.399

0.371
  pilB Haemophilus influenzae Rd KW20

37.356

96.399

0.36

Multiple sequence alignment