Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   FGL04_RS00650 Genome accession   NZ_LR594035
Coordinates   102683..103084 (+) Length   133 a.a.
NCBI ID   WP_171011213.1    Uniprot ID   -
Organism   Streptococcus pseudoporcinus strain NCTC5385     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97683..108084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL04_RS00630 (NCTC5385_00128) - 101434..101805 (+) 372 WP_225247737.1 DUF1033 family protein -
  FGL04_RS00635 (NCTC5385_00129) comGC 101744..102010 (+) 267 WP_138067846.1 competence type IV pilus major pilin ComGC Machinery gene
  FGL04_RS00640 (NCTC5385_00130) comGD/cglD 101970..102398 (+) 429 WP_037598912.1 competence type IV pilus minor pilin ComGD Machinery gene
  FGL04_RS00645 comGE 102451..102663 (+) 213 WP_229296016.1 competence system putative prepilin ComGE Machinery gene
  FGL04_RS00650 (NCTC5385_00131) comGF/cglF 102683..103084 (+) 402 WP_171011213.1 competence type IV pilus minor pilin ComGF Machinery gene
  FGL04_RS00655 (NCTC5385_00132) comGG 103062..103463 (+) 402 WP_138067849.1 competence type IV pilus minor pilin ComGG -
  FGL04_RS00660 (NCTC5385_00133) comGH 103508..104464 (+) 957 WP_138067850.1 class I SAM-dependent methyltransferase Machinery gene
  FGL04_RS00665 (NCTC5385_00134) - 104520..105713 (+) 1194 WP_138067851.1 acetate kinase -
  FGL04_RS00670 (NCTC5385_00135) - 106076..106285 (+) 210 WP_007895522.1 helix-turn-helix transcriptional regulator -
  FGL04_RS00675 (NCTC5385_00136) - 106282..106734 (+) 453 WP_138067852.1 hypothetical protein -
  FGL04_RS00680 (NCTC5385_00137) - 106746..107393 (+) 648 WP_138067853.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 133 a.a.        Molecular weight: 15543.90 Da        Isoelectric Point: 9.9973

>NTDB_id=1005000 FGL04_RS00650 WP_171011213.1 102683..103084(+) (comGF/cglF) [Streptococcus pseudoporcinus strain NCTC5385]
MESLIALMVISGTLLVYQGLSHSLLSHMTYLRKSDQDKWLLFTHQFRSECIGARFRSVRGNQLYIEKDGKELAFGYSSKK
DFRKASAQWHGYHPMLLNISSAHISEQDQRIRLVLKWPSGLERTFIYDFKEKS

Nucleotide


Download         Length: 402 bp        

>NTDB_id=1005000 FGL04_RS00650 WP_171011213.1 102683..103084(+) (comGF/cglF) [Streptococcus pseudoporcinus strain NCTC5385]
ATGGAATCATTAATTGCTTTAATGGTCATTTCAGGAACACTTTTGGTTTACCAAGGACTATCGCACTCTCTTTTATCCCA
CATGACCTATTTAAGAAAAAGTGATCAGGACAAATGGCTACTGTTTACTCATCAATTTCGTTCAGAATGCATAGGAGCGC
GTTTTCGGAGTGTTCGAGGCAATCAGCTTTATATCGAAAAAGACGGTAAAGAACTAGCTTTTGGCTATAGCAGCAAAAAA
GATTTTCGGAAGGCTTCGGCGCAATGGCACGGTTACCATCCCATGTTACTTAATATCTCGAGTGCCCATATCTCTGAACA
GGATCAACGAATAAGACTTGTGTTAAAATGGCCGAGTGGCTTGGAAAGGACCTTTATTTATGATTTTAAGGAAAAATCTT
AA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(30-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae TIGR4

45.522

100

0.459
  comGF/cglF Streptococcus pneumoniae Rx1

45.522

100

0.459
  comGF/cglF Streptococcus pneumoniae D39

45.522

100

0.459
  comGF/cglF Streptococcus pneumoniae R6

45.522

100

0.459
  comGF/cglF Streptococcus mitis NCTC 12261

45.669

95.489

0.436
  comGF Streptococcus mutans UA159

45.6

93.985

0.429
  comGF Streptococcus mutans UA140

44.8

93.985

0.421
  comGF/cglF Streptococcus mitis SK321

43.307

95.489

0.414
  comGF Lactococcus lactis subsp. cremoris KW2

37.405

98.496

0.368