Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AAEY33_RS18485 Genome accession   NZ_CP157082
Coordinates   3798672..3799748 (-) Length   358 a.a.
NCBI ID   WP_098370095.1    Uniprot ID   -
Organism   Peribacillus simplex strain IMGN11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3793672..3804748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEY33_RS18445 (AAEY33_18440) - 3794533..3795333 (+) 801 WP_347939906.1 YqhG family protein -
  AAEY33_RS18450 (AAEY33_18445) - 3795380..3795565 (-) 186 WP_347939907.1 YqzE family protein -
  AAEY33_RS18455 (AAEY33_18450) comGG 3795683..3796069 (-) 387 WP_179890981.1 competence type IV pilus minor pilin ComGG -
  AAEY33_RS18460 (AAEY33_18455) comGF 3796062..3796541 (-) 480 WP_098369820.1 competence type IV pilus minor pilin ComGF -
  AAEY33_RS18465 (AAEY33_18460) - 3796489..3796827 (-) 339 WP_141993837.1 hypothetical protein -
  AAEY33_RS18470 (AAEY33_18465) comGD 3796814..3797257 (-) 444 WP_144526704.1 competence type IV pilus minor pilin ComGD -
  AAEY33_RS18475 (AAEY33_18470) comGC 3797265..3797576 (-) 312 WP_063233178.1 competence type IV pilus major pilin ComGC -
  AAEY33_RS18480 (AAEY33_18475) comGB 3797654..3798706 (-) 1053 WP_347939908.1 competence type IV pilus assembly protein ComGB -
  AAEY33_RS18485 (AAEY33_18480) comGA 3798672..3799748 (-) 1077 WP_098370095.1 competence type IV pilus ATPase ComGA Machinery gene
  AAEY33_RS18490 (AAEY33_18485) - 3799856..3800236 (-) 381 WP_347939909.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AAEY33_RS18495 (AAEY33_18490) - 3800449..3801150 (+) 702 WP_144526701.1 helix-turn-helix domain-containing protein -
  AAEY33_RS18500 (AAEY33_18495) - 3801236..3801478 (+) 243 WP_076365317.1 DUF2626 domain-containing protein -
  AAEY33_RS18505 (AAEY33_18500) - 3801531..3802631 (-) 1101 WP_347939910.1 SAM-dependent methyltransferase -
  AAEY33_RS18510 (AAEY33_18505) - 3803031..3803657 (-) 627 WP_098369813.1 MBL fold metallo-hydrolase -
  AAEY33_RS18515 (AAEY33_18510) - 3803891..3804064 (+) 174 WP_034308396.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39772.38 Da        Isoelectric Point: 8.4662

>NTDB_id=1004013 AAEY33_RS18485 WP_098370095.1 3798672..3799748(-) (comGA) [Peribacillus simplex strain IMGN11]
MISIEKTAEKILTRAVQESASDIHIFFRKEGPLIQFRIDNKLVPKETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPSTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
AHEMINRNIITLEDPIENVSEKVLQVQINEKAGVTYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEVEQSLIGVTAQRLVELRCLPCKGDCDLACKMTARNKRASVYELLYGKSLAEVLRIMGDEK
GKATVSYRQLKDEIGKAVAMGYVDSGEYERLVYDETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=1004013 AAEY33_RS18485 WP_098370095.1 3798672..3799748(-) (comGA) [Peribacillus simplex strain IMGN11]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTGACCCGTGCTGTACAGGAATCGGCATCGGATATCCACATTTTTTT
TCGCAAGGAGGGACCTCTCATCCAATTCAGGATAGACAATAAGCTTGTTCCAAAGGAAACATTATCATTCTTTGAAGCAG
AGAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGTGCCATCACC
ATCAATTTGGCCAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAGCACCGTTCAAAAATTGATTGCTCTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACTGGCAGTGGAAAAACCACGACACTATATTCCCTGCTTCACCAT
GCCCATGAGATGATCAATCGAAATATTATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAGGTATTGCAAGTCCA
AATCAATGAAAAGGCGGGCGTTACGTATTCTGTCGGTCTAAAAGCTGTTCTGAGGCATGACCCTGACGTGATCATGGTTG
GGGAAGTCAGAGATGCAGAAACCGCCAAAATCGCAGTGCGTGCCGCATTGACCGGTCATTTGATACTTACAACCATGCAT
ACCAGGGACGCCCAGGGCGCCATCTCCAGGTTACTGGAATTTGGTGTCAGCCTGCTTGAGGTTGAACAGAGTTTGATTGG
CGTGACAGCACAGCGGCTGGTTGAATTGCGATGTCTTCCATGTAAAGGGGACTGTGATTTAGCTTGCAAAATGACTGCCA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGAAAAAGCCTGGCTGAGGTTCTCCGGATAATGGGAGATGAAAAA
GGAAAGGCAACGGTCAGCTACCGCCAATTGAAGGATGAAATCGGAAAAGCGGTTGCGATGGGATATGTGGATTCCGGGGA
ATATGAACGGCTGGTATACGATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.022

99.721

0.559
  pilB Haemophilus influenzae 86-028NP

39.031

98.045

0.383
  pilB Haemophilus influenzae Rd KW20

37.607

98.045

0.369

Multiple sequence alignment