Detailed information NTG1003663

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EL305_RS09255
• Genome accession: NZ_LR134528
• Coordinates: 1543488..1544714 (-)
• Length: 408 a.a.
• NCBI ID: WP_002218483.1
• Uniprot ID: Q9K1N4
• Organism: Neisseria meningitidis strain NCTC12163
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1538488..1549714

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL305_RS09230 (NCTC12163_01539)	-	1538778..1539785 (+)	1008	WP_002250644.1	IS5 family transposase	-
EL305_RS09250 (NCTC12163_01540)	yccS	1541052..1543202 (-)	2151	WP_126418079.1	YccS family putative transporter	-
EL305_RS09255 (NCTC12163_01541)	pilU	1543488..1544714 (-)	1227	WP_002218483.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EL305_RS09260 (NCTC12163_01542)	pilT	1544877..1545920 (-)	1044	WP_126418081.1	type IV pilus twitching motility protein PilT	Machinery gene
EL305_RS09265 (NCTC12163_01543)	-	1546040..1546726 (+)	687	WP_002231428.1	YggS family pyridoxal phosphate-dependent enzyme	-
EL305_RS09270	-	1546726..1547134 (+)	409	Protein_1556	RNA-binding protein	-
EL305_RS09275 (NCTC12163_01544)	proC	1547183..1547974 (+)	792	WP_002215263.1	pyrroline-5-carboxylate reductase	-
EL305_RS09280 (NCTC12163_01545)	dksA	1548121..1548537 (+)	417	WP_002215267.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45674.28 Da
• Isoelectric Point: 6.9640

>NTDB_id=1003663 EL305_RS09255 WP_002218483.1 1543488..1544714(-) (pilU) [Neisseria meningitidis strain NCTC12163]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=1003663 EL305_RS09255 WP_002218483.1 1543488..1544714(-) (pilU) [Neisseria meningitidis strain NCTC12163]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q9K1N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.598	88.48	0.395
pilU	Pseudomonas stutzeri DSM 10701	44.663	87.255	0.39
pilU	Vibrio cholerae strain A1552	46.429	82.353	0.382