Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   AACH30_RS04100 Genome accession   NZ_AP027243
Coordinates   817894..819150 (-) Length   418 a.a.
NCBI ID   WP_265150686.1    Uniprot ID   A0AA43T877
Organism   Lactococcus garvieae strain L21-68     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 812894..824150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH30_RS04065 (LG21E68_07970) rpmA 813014..813298 (+) 285 WP_004257956.1 50S ribosomal protein L27 -
  AACH30_RS04070 (LG21E68_07980) ald 813649..814755 (+) 1107 WP_265150684.1 alanine dehydrogenase -
  AACH30_RS04075 (LG21E68_07990) - 814873..815832 (+) 960 WP_004257953.1 PhoH family protein -
  AACH30_RS04080 (LG21E68_08000) - 815834..816307 (+) 474 WP_265150685.1 NUDIX hydrolase -
  AACH30_RS04085 (LG21E68_08010) ybeY 816317..816805 (+) 489 WP_014024619.1 rRNA maturation RNase YbeY -
  AACH30_RS04090 (LG21E68_08020) - 816789..817250 (+) 462 WP_176490186.1 diacylglycerol kinase family protein -
  AACH30_RS04095 (LG21E68_08030) - 817253..817897 (-) 645 WP_176490185.1 ComF family protein -
  AACH30_RS04100 (LG21E68_08040) comFA 817894..819150 (-) 1257 WP_265150686.1 DEAD/DEAH box helicase Machinery gene
  AACH30_RS04105 (LG21E68_08050) - 819205..819828 (+) 624 WP_004257938.1 YigZ family protein -
  AACH30_RS04110 (LG21E68_08060) - 819842..820864 (+) 1023 WP_023889525.1 DUF475 domain-containing protein -
  AACH30_RS04115 (LG21E68_08070) - 820894..821916 (-) 1023 WP_265150687.1 alpha/beta hydrolase -
  AACH30_RS04120 (LG21E68_08080) - 822235..823014 (+) 780 WP_019292766.1 formate/nitrite transporter family protein -
  AACH30_RS04125 (LG21E68_08090) glyQ 823028..823996 (+) 969 WP_004257927.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47919.70 Da        Isoelectric Point: 9.9188

>NTDB_id=100314 AACH30_RS04100 WP_265150686.1 817894..819150(-) (comFA) [Lactococcus garvieae strain L21-68]
MDELYGRLLLQKELTKLPDKAILFDGMQDVSQTVMMCNRCGKKIKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FSAASFLNWQGKLTASQQHVSDSLVNLHQQQKTILVQAVTGAGKTEMIYQSINNTLKKGKAVGLTSPRIDVCLELYHRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLILDEVDAFPFPDNEMLYFALDQARKPSSSLIYLTATTTDN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRESGFPLLIFVPEIRQGEKLCQDLQSNFPHEEIAFVAS
TSLERLEAVERFRKGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPEGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFL

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=100314 AACH30_RS04100 WP_265150686.1 817894..819150(-) (comFA) [Lactococcus garvieae strain L21-68]
ATGGATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGACGAAACTTCCAGATAAAGCAATACTTTTTGATGGGAT
GCAAGATGTTTCCCAAACAGTGATGATGTGCAACCGCTGTGGCAAAAAGATTAAGAAAAAAGAGGTCCTTCTCCCTGTTG
GTGCTTATTATTGTCCTCATTGTATTCAAATGGGACGTGTTCGCTCGGACGAAAAACTTTACCACCTTCCACAGGAGGAT
TTTTCAGCAGCTTCTTTTCTCAATTGGCAGGGAAAACTCACAGCATCTCAGCAACATGTATCCGATAGCCTAGTCAATTT
GCATCAGCAACAAAAAACAATACTGGTACAGGCGGTTACTGGTGCAGGAAAAACTGAAATGATTTATCAGAGCATTAATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGCCCGCGTATTGATGTCTGTCTAGAGCTCTATCATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTGCACGGAAAGAGTGAAAAATACAGCCGCTCGCCACTCGTAATTGCAACGAC
ACATCAGCTTATGCGTTTTCGCCATGCCTTTGACTTACTCATTCTTGATGAGGTGGATGCCTTTCCCTTTCCAGACAATG
AAATGCTTTATTTTGCACTGGATCAAGCTCGTAAACCTTCTTCAAGTTTGATTTATCTTACTGCAACCACCACCGATAAT
TTGGAAAAACAAGTCAAATTAGGACAAATCGAAAAACTACAGCTTCCACGACGTTTTCATGGTTTTCCTCTGGTACTCCC
GCAATTTTTTTGGCAAAGCAAGTTTTACAAAATGGTCAAAAAACAACGCGAGTCTGGTTTTCCCTTACTTATCTTTGTCC
CTGAAATAAGACAGGGAGAAAAACTCTGCCAAGATTTACAGAGTAATTTTCCTCATGAAGAAATTGCTTTTGTCGCGTCA
ACGAGTCTTGAACGCTTAGAGGCTGTGGAACGCTTTCGTAAGGGCAATATTTCTATCCTTGTCTCAACGACCATTTTAGA
ACGTGGTGTAACTTTCCCTAAGGTTGACGTTTTTGTGTTTCAAAGTCACCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGAGGGTAAGGTATTTTTCTTTCATTTAGGAAAAACTACAGCG
ATGTTGGAAGCCTATAAAAATATCAGAAATATGAATAAAGCAGGAGGGTTCTTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(274-382)

Type III restriction enzyme, res subunit (ResIII)

(92-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.393

95.455

0.548
  comFA/cflA Streptococcus mitis SK321

52.347

100

0.533
  comFA/cflA Streptococcus pneumoniae TIGR4

52.113

100

0.531
  comFA/cflA Streptococcus pneumoniae Rx1

52.113

100

0.531
  comFA/cflA Streptococcus pneumoniae D39

52.113

100

0.531
  comFA/cflA Streptococcus pneumoniae R6

52.113

100

0.531
  comFA/cflA Streptococcus mitis NCTC 12261

51.174

100

0.522
  comFA Bacillus subtilis subsp. subtilis str. 168

39.043

94.976

0.371
  comFA Latilactobacillus sakei subsp. sakei 23K

38.539

94.976

0.366

Multiple sequence alignment