Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABEF90_RS12465 Genome accession   NZ_CP156793
Coordinates   2561020..2561811 (-) Length   263 a.a.
NCBI ID   WP_161788388.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7914     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2556020..2566811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF90_RS12445 (ABEF90_12445) - 2557068..2557766 (-) 699 WP_347453145.1 tetratricopeptide repeat protein -
  ABEF90_RS12450 (ABEF90_12450) hisS 2557797..2559092 (-) 1296 WP_347474022.1 histidine--tRNA ligase -
  ABEF90_RS12455 (ABEF90_12455) ispG 2559096..2560217 (-) 1122 WP_347454714.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABEF90_RS12460 (ABEF90_12460) - 2560241..2561029 (-) 789 WP_347473387.1 RodZ domain-containing protein -
  ABEF90_RS12465 (ABEF90_12465) pilF 2561020..2561811 (-) 792 WP_161788388.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABEF90_RS12470 (ABEF90_12470) rlmN 2561835..2563070 (-) 1236 WP_347473386.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABEF90_RS12475 (ABEF90_12475) ndk 2563193..2563624 (-) 432 WP_034587246.1 nucleoside-diphosphate kinase -
  ABEF90_RS12480 (ABEF90_12480) iscX 2563735..2563935 (-) 201 WP_004813114.1 Fe-S cluster assembly protein IscX -
  ABEF90_RS12485 (ABEF90_12485) - 2564023..2564577 (-) 555 WP_347454179.1 phosphatase PAP2 family protein -
  ABEF90_RS12490 (ABEF90_12490) - 2564671..2565081 (-) 411 WP_347454710.1 hypothetical protein -

Sequence

Protein


Download         Length: 263 a.a.        Molecular weight: 29698.87 Da        Isoelectric Point: 9.2335

>NTDB_id=1002857 ABEF90_RS12465 WP_161788388.1 2561020..2561811(-) (pilF) [Acinetobacter thermotolerans strain ANC 7914]
MTSKIALISTVCFSLALTACQTVDAGKKDPEKAIKVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVLLQQ
EGSKANLEKAESYFKKAISAEPKNAQARNNYGTYLYQLERYNDAIEQFEIAGATLGYDQRYRALENLGRIYLKKGDLVNA
EKSFKQALQANRDSYISMLELAEIFYLNQQFPAASKMYEQFVHAVGQKNQGARALWIGIRTARASGDQLGMQVLVNQLRA
LFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 792 bp        

>NTDB_id=1002857 ABEF90_RS12465 WP_161788388.1 2561020..2561811(-) (pilF) [Acinetobacter thermotolerans strain ANC 7914]
ATGACATCTAAAATTGCTTTAATTTCAACAGTATGTTTTTCACTTGCATTGACTGCCTGTCAGACGGTAGACGCGGGGAA
AAAGGACCCGGAAAAGGCAATTAAGGTACGTACTCAATTGGCGGTTGAATACCTGAAAACAGGTGATCTGGATTCAGCCA
AGCGTGCACTGGACGAAGCACTGAAAATCAATTCACGTGATGCCCAGGCCAATATGATGATGGGTGTGCTGCTCCAGCAG
GAAGGCAGTAAGGCTAATCTGGAAAAAGCGGAAAGTTATTTTAAAAAAGCGATTTCTGCTGAACCGAAAAATGCACAGGC
GCGTAACAATTACGGCACCTATTTATATCAACTTGAACGTTATAATGACGCGATTGAACAGTTTGAAATTGCCGGTGCAA
CCCTGGGTTATGACCAGCGTTACCGTGCCCTGGAAAATCTGGGGCGGATCTACCTGAAAAAAGGGGATCTGGTAAATGCT
GAAAAATCATTCAAACAGGCACTACAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAGATTTTTTATCT
GAACCAGCAGTTCCCTGCAGCGAGCAAAATGTATGAACAGTTTGTGCATGCGGTAGGGCAGAAGAATCAGGGTGCCCGCG
CACTCTGGATAGGCATTCGCACAGCGCGTGCCAGTGGCGATCAACTCGGAATGCAGGTACTGGTGAATCAGCTGCGTGCA
CTTTTCCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(94-126)

Tetratricopeptide repeat (TPR_8)

(35-65)

Tetratricopeptide repeat (TPR_8)

(142-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.846

98.859

0.779
  pilF Acinetobacter baylyi ADP1

69.804

96.958

0.677

Multiple sequence alignment