Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   AACH63_RS04085 Genome accession   NZ_AP027240
Coordinates   809253..810509 (-) Length   418 a.a.
NCBI ID   WP_096368377.1    Uniprot ID   -
Organism   Lactococcus formosensis strain L21-20     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 804253..815509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH63_RS04055 (LG21E20_08040) - 805007..806113 (+) 1107 WP_096368381.1 alanine dehydrogenase -
  AACH63_RS04060 (LG21E20_08050) - 806232..807191 (+) 960 WP_096368380.1 PhoH family protein -
  AACH63_RS04065 (LG21E20_08060) - 807193..807666 (+) 474 WP_017369708.1 NUDIX hydrolase -
  AACH63_RS04070 (LG21E20_08070) ybeY 807676..808164 (+) 489 WP_014024619.1 rRNA maturation RNase YbeY -
  AACH63_RS04075 (LG21E20_08080) - 808148..808609 (+) 462 WP_096368379.1 diacylglycerol kinase family protein -
  AACH63_RS04080 (LG21E20_08090) - 808612..809256 (-) 645 WP_096368378.1 ComF family protein -
  AACH63_RS04085 (LG21E20_08100) comFA 809253..810509 (-) 1257 WP_096368377.1 DEAD/DEAH box helicase Machinery gene
  AACH63_RS04090 (LG21E20_08110) - 810564..811187 (+) 624 WP_096368376.1 YigZ family protein -
  AACH63_RS04095 (LG21E20_08120) - 811200..812222 (+) 1023 WP_019293557.1 DUF475 domain-containing protein -
  AACH63_RS04100 - 812259..813280 (-) 1022 Protein_801 alpha/beta hydrolase -
  AACH63_RS04105 (LG21E20_08150) - 813598..814377 (+) 780 WP_096368375.1 formate/nitrite transporter family protein -
  AACH63_RS04110 (LG21E20_08160) glyQ 814391..815359 (+) 969 WP_019292765.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47857.06 Da        Isoelectric Point: 10.3108

>NTDB_id=100269 AACH63_RS04085 WP_096368377.1 809253..810509(-) (comFA) [Lactococcus formosensis strain L21-20]
MNELYGRLLLQKELTKIPDKAILFDGMKEVSKTIVMCNRCGKKSKKKEVYLPVGAYYCPHCIQMGRVRSDEKLYHLPQKN
FAAVSCLKWQGKLTGPQQYISDNLLNLHRQQKTVLVQAVTGAGKTEMIYQVIDATLKKGKAVGLTSPRIDVCLELYYRLK
RNFSCPISLLHGKGEKYSRSPLVIATTHQLLRFRHAFDLLILDEVDAFPFPDNEMLYFALAQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRKSGFPLLIFVPEIRLGEKLCQDLQTYFPHEEIAFVAS
TSLERLASVERFRQGKVSILVSTTILERGVTFPKVDVFVFQSHHHNFTSSSLIQIAGRVGRSTERPDGKVFFFHLGKTTA
MLEANKNIRNMNKAGEFL

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=100269 AACH63_RS04085 WP_096368377.1 809253..810509(-) (comFA) [Lactococcus formosensis strain L21-20]
ATGAATGAATTATACGGTAGACTTCTTCTGCAAAAAGAATTGACGAAGATACCGGACAAAGCCATACTTTTTGATGGTAT
GAAAGAAGTTTCAAAAACGATTGTCATGTGTAACCGCTGTGGTAAAAAGAGTAAGAAAAAAGAGGTTTATCTCCCCGTTG
GTGCTTATTATTGCCCCCATTGTATTCAGATGGGGCGTGTTCGCTCTGATGAAAAACTTTATCACCTTCCACAGAAAAAT
TTTGCTGCAGTTTCTTGTCTAAAATGGCAGGGAAAGCTCACAGGTCCTCAGCAATATATCTCCGATAATTTACTTAATCT
GCATCGGCAGCAAAAAACAGTGCTGGTACAGGCTGTAACAGGGGCAGGAAAGACGGAAATGATTTATCAAGTCATTGATG
CCACCTTAAAGAAAGGAAAAGCTGTGGGTTTGACGAGTCCGCGTATTGATGTTTGTCTAGAACTTTACTATCGCTTAAAG
AGGAATTTTTCCTGTCCCATCTCTCTTTTGCATGGAAAGGGTGAGAAATACAGCCGTTCCCCTCTTGTAATTGCAACCAC
ACATCAGCTTTTACGTTTTCGTCATGCCTTTGATTTACTGATTCTCGACGAAGTTGACGCTTTTCCATTTCCAGATAATG
AAATGCTTTACTTTGCGCTGGCTCAAGCTCGAAAACCCTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAACAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGCTGCAACTTCCCCGACGCTTTCATGGTTTTCCACTAGTACTTCC
GCAATTTTTTTGGCAGAGCAAGTTTTACAAGATGGTCAAAAAACAACGAAAATCTGGTTTTCCATTACTTATCTTTGTCC
CTGAAATAAGACTAGGAGAAAAACTGTGCCAAGACTTACAAACCTATTTTCCTCATGAAGAAATTGCCTTTGTTGCATCA
ACGAGTCTTGAACGCTTAGCATCTGTGGAACGTTTTCGACAAGGAAAAGTTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACCTTCCCTAAGGTTGACGTTTTTGTATTTCAAAGTCACCATCATAATTTCACAAGTTCAAGTCTCATTC
AAATTGCTGGACGAGTAGGACGAAGTACTGAAAGACCTGATGGTAAGGTATTTTTCTTTCATTTAGGAAAAACAACAGCG
ATGTTGGAAGCCAATAAAAACATCAGAAATATGAATAAAGCTGGAGAATTCCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(275-382)

Type III restriction enzyme, res subunit (ResIII)

(93-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.644

95.455

0.55
  comFA/cflA Streptococcus pneumoniae TIGR4

52.358

100

0.531
  comFA/cflA Streptococcus pneumoniae Rx1

52.358

100

0.531
  comFA/cflA Streptococcus pneumoniae D39

52.358

100

0.531
  comFA/cflA Streptococcus pneumoniae R6

52.358

100

0.531
  comFA/cflA Streptococcus mitis SK321

51.887

100

0.526
  comFA/cflA Streptococcus mitis NCTC 12261

50.943

100

0.517
  comFA Bacillus subtilis subsp. subtilis str. 168

37.9

100

0.397
  comFA Latilactobacillus sakei subsp. sakei 23K

38.25

95.694

0.366

Multiple sequence alignment