Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   AACH29_RS03970 Genome accession   NZ_AP027239
Coordinates   801129..802385 (-) Length   418 a.a.
NCBI ID   WP_014024622.1    Uniprot ID   F9VD58
Organism   Lactococcus garvieae strain L21-12     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 796129..807385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH29_RS03935 (LG21E12_07720) rpmA 796250..796534 (+) 285 WP_004257956.1 50S ribosomal protein L27 -
  AACH29_RS03940 (LG21E12_07730) - 796884..797990 (+) 1107 WP_338659912.1 alanine dehydrogenase -
  AACH29_RS03945 (LG21E12_07740) - 798108..799067 (+) 960 WP_004257953.1 PhoH family protein -
  AACH29_RS03950 (LG21E12_07750) - 799069..799542 (+) 474 WP_014024618.1 NUDIX hydrolase -
  AACH29_RS03955 (LG21E12_07760) ybeY 799552..800040 (+) 489 WP_014024619.1 rRNA maturation RNase YbeY -
  AACH29_RS03960 (LG21E12_07770) - 800024..800485 (+) 462 WP_014024620.1 diacylglycerol kinase family protein -
  AACH29_RS03965 (LG21E12_07780) - 800488..801132 (-) 645 WP_014024621.1 ComF family protein -
  AACH29_RS03970 (LG21E12_07790) comFA 801129..802385 (-) 1257 WP_014024622.1 DEAD/DEAH box helicase Machinery gene
  AACH29_RS03975 (LG21E12_07800) - 802440..803063 (+) 624 WP_004257938.1 YigZ family protein -
  AACH29_RS03980 (LG21E12_07810) - 803077..804099 (+) 1023 WP_014024623.1 DUF475 domain-containing protein -
  AACH29_RS03985 (LG21E12_07820) - 804131..805153 (-) 1023 WP_014024624.1 alpha/beta hydrolase -
  AACH29_RS03990 (LG21E12_07830) - 805472..806251 (+) 780 WP_014024625.1 formate/nitrite transporter family protein -
  AACH29_RS03995 (LG21E12_07840) glyQ 806265..807233 (+) 969 WP_004257927.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47953.80 Da        Isoelectric Point: 9.9109

>NTDB_id=100224 AACH29_RS03970 WP_014024622.1 801129..802385(-) (comFA) [Lactococcus garvieae strain L21-12]
MDELYGRLLLQKELTKLPDKATLFDGMQDVSKTVMMCNRCGKKIKKKEVLLPVGAYYCPHCIQMGRVRSDEKLYHLPQED
FSAASFLNWQGKLTASQQHVSDSLVKLQQQQKTVLVQAVTGAGKTEMIYQSIDNTLKKGKAVGLTSPRIDVCLELYHRLK
RDFSCPISLLHGKSEKYSRSPLVIATTHQLMRFRHAFDLLILDEVDAFPFPDNEMLYFELAQARKPSSILIYLTATTTDN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMIKKQRESGFPLLIFVPEIRQGEKLCQDLQSNFPHEEIAFVAS
TSLERLEAVERFRQGNISILVSTTILERGVTFPKVDVFVFQSHHHNFTRSSLIQIAGRVGRSTERPEGKVFFFHLGKTTA
MLEAYKNIRNMNKAGGFL

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=100224 AACH29_RS03970 WP_014024622.1 801129..802385(-) (comFA) [Lactococcus garvieae strain L21-12]
ATGGATGAATTATATGGTAGACTCCTTTTACAAAAAGAATTGACGAAACTTCCAGATAAAGCAACACTTTTTGATGGGAT
GCAAGATGTTTCCAAAACAGTGATGATGTGCAACCGCTGTGGCAAAAAGATCAAGAAAAAAGAGGTCCTTCTCCCTGTTG
GTGCTTATTATTGTCCCCATTGTATTCAAATGGGACGTGTTCGCTCGGATGAAAAACTTTACCACCTTCCCCAGGAGGAT
TTTTCAGCAGCTTCTTTTCTCAACTGGCAGGGAAAACTCACAGCATCTCAGCAACATGTATCCGATAGCCTAGTCAAATT
GCAACAGCAACAAAAAACAGTACTGGTACAGGCGGTTACTGGTGCAGGAAAAACCGAAATGATTTATCAGAGCATTGATA
ACACCCTTAAAAAAGGAAAAGCTGTAGGTTTAACTAGCCCGCGTATTGATGTCTGTCTAGAGCTCTATCATCGCTTAAAG
AGAGATTTTTCTTGTCCCATCTCGCTTTTGCACGGAAAGAGTGAAAAATACAGCCGCTCGCCACTCGTAATCGCAACGAC
ACATCAGCTTATGCGTTTTCGCCATGCCTTTGACTTACTCATTCTTGATGAGGTGGATGCCTTTCCCTTTCCAGACAATG
AAATGCTTTATTTTGAGCTGGCTCAAGCCCGTAAGCCTTCTTCAATCTTGATTTATCTTACTGCAACCACCACCGATAAT
TTGGAAAAACAAGTCAAGTTAGGACAAATCGAGAAGCTACAGCTTCCACGACGTTTTCATGGTTTTCCTCTGGTACTCCC
GCAATTTTTTTGGCAAAGCAAATTTTACAAAATGATCAAAAAACAACGCGAGTCTGGTTTTCCCTTACTTATCTTTGTTC
CTGAAATAAGACAGGGAGAAAAACTCTGCCAAGATTTACAGAGTAATTTTCCTCATGAAGAAATTGCTTTTGTCGCGTCA
ACAAGTCTTGAACGCTTAGAGGCTGTGGAACGCTTTCGTCAGGGCAATATTTCTATCCTTGTCTCAACGACCATTTTAGA
ACGTGGTGTAACTTTCCCTAAGGTTGACGTTTTTGTGTTTCAAAGTCACCACCATAATTTCACAAGATCAAGCCTCATCC
AAATTGCCGGACGAGTAGGTCGAAGTACCGAAAGACCTGAGGGTAAGGTATTTTTCTTTCATTTAGGAAAAACTACAGCG
ATGTTGGAAGCCTATAAAAATATCAGAAATATGAATAAAGCAGGAGGGTTCCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(274-382)

Type III restriction enzyme, res subunit (ResIII)

(92-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F9VD58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.398

93.78

0.538
  comFA/cflA Streptococcus mitis SK321

52.347

100

0.533
  comFA/cflA Streptococcus pneumoniae TIGR4

52.347

100

0.533
  comFA/cflA Streptococcus pneumoniae Rx1

52.347

100

0.533
  comFA/cflA Streptococcus pneumoniae D39

52.347

100

0.533
  comFA/cflA Streptococcus pneumoniae R6

52.347

100

0.533
  comFA/cflA Streptococcus mitis NCTC 12261

51.408

100

0.524
  comFA Bacillus subtilis subsp. subtilis str. 168

38.366

96.651

0.371
  comFA Latilactobacillus sakei subsp. sakei 23K

38.287

94.976

0.364

Multiple sequence alignment