Detailed information of TA system TA095239

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1718403..1718623
• Replicon: chromosome
• Accession: NZ_CP026599
• Organism: Clostridioides difficile strain 09-00072

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: C3353_RS08335
• Protein ID: WP_003429855.1
• Coordinates: 1718403..1718564 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1718484..1718623 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3353_RS08300	1714260..1714697	+	438	WP_009888839.1	hypothetical protein	-
C3353_RS08305	1714690..1714866	+	177	WP_009888840.1	hypothetical protein	-
C3353_RS08310	1714867..1716177	+	1311	WP_009893136.1	phage tail sheath family protein	-
C3353_RS08315	1716194..1716664	+	471	WP_009888842.1	phage tail tube protein	-
C3353_RS08320	1716723..1717550	+	828	WP_009888843.1	hypothetical protein	-
C3353_RS08325	1717622..1718062	+	441	WP_003429853.1	phage portal protein	-
C3353_RS08335	1718403..1718564	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1718484..1718623	-	140	NuclAT_1	-	Antitoxin
-	1718484..1718623	-	140	NuclAT_2	-	Antitoxin
-	1718968..1719016	-	49	NuclAT_4	-	-
-	1718968..1719016	-	49	NuclAT_5	-	-
C3353_RS08340	1719901..1720089	+	189	WP_003429858.1	hypothetical protein	-
C3353_RS08345	1720208..1721074	+	867	WP_009888845.1	hypothetical protein	-
C3353_RS08355	1721939..1722466	+	528	WP_009888847.1	DUF4352 domain-containing protein	-
C3353_RS08360	1722604..1723371	+	768	WP_009888848.1	DUF4428 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_013316 Clostridium difficile R20291 Prophage TA024266 1671047..1738184 0	inside	Prophage	-	-	1684614..1751051	66437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T95239 WP_003429855.1 NZ_CP026599:1718403-1718564 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T95239 NZ_CP026599:1718403-1718564 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCACAAATTTAAAT
AA

Antitoxin

Nucleotide

• Length: 140 bp

>AT95239 NZ_CP026599:c1718623-1718484 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGACAGTAGAGTGAAGTTCATAATTTAAACAATTAGTAATTATTTAAATTTGTGGAACTT
GATTTTAAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin