Detailed information of TA system TA095231

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1718441..1718661
• Replicon: chromosome
• Accession: NZ_CP026597
• Organism: Clostridioides difficile strain 10-00078

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: C3351_RS08350
• Protein ID: WP_003429855.1
• Coordinates: 1718441..1718602 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1718522..1718661 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3351_RS08315	1714299..1714736	+	438	WP_009888839.1	hypothetical protein	-
C3351_RS08320	1714729..1714905	+	177	WP_009888840.1	hypothetical protein	-
C3351_RS08325	1714906..1716216	+	1311	WP_009893136.1	phage tail sheath family protein	-
C3351_RS08330	1716233..1716702	+	470	Protein_1505	phage tail tube protein	-
C3351_RS08335	1716761..1717588	+	828	WP_009888843.1	hypothetical protein	-
C3351_RS08340	1717660..1718100	+	441	WP_003429853.1	phage portal protein	-
C3351_RS08350	1718441..1718602	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1718522..1718661	-	140	NuclAT_1	-	Antitoxin
-	1718522..1718661	-	140	NuclAT_2	-	Antitoxin
-	1719006..1719054	-	49	NuclAT_4	-	-
-	1719006..1719054	-	49	NuclAT_5	-	-
C3351_RS08355	1719939..1720127	+	189	WP_003429858.1	hypothetical protein	-
C3351_RS08360	1720246..1721112	+	867	WP_009888845.1	hypothetical protein	-
C3351_RS08370	1721977..1722504	+	528	WP_009888847.1	DUF4352 domain-containing protein	-
C3351_RS08375	1722642..1723409	+	768	WP_009888848.1	DUF4428 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_013316 Clostridium difficile R20291 Prophage TA024266 1671047..1738184 0	inside	Prophage	-	-	1684653..1751789	67136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T95231 WP_003429855.1 NZ_CP026597:1718441-1718602 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T95231 NZ_CP026597:1718441-1718602 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCACAAATTTAAAT
AA

Antitoxin

Nucleotide

• Length: 140 bp

>AT95231 NZ_CP026597:c1718661-1718522 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGACAGTAGAGTGAAGTTCATAATTTAAACAATTAGTAATTATTTAAATTTGTGGAACTT
GATTTTAAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin