Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) CD-RCd/-
Location 2419057..2419259 Replicon chromosome
Accession NZ_CP025047
Organism Clostridioides difficile strain W0003a

Toxin (Protein)


Gene name CD2299.1 Uniprot ID Q185H1
Locus tag CWR54_RS11305 Protein ID WP_004454589.1
Coordinates 2419107..2419259 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name SQ1641
Locus tag -
Coordinates 2419057..2419186 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWR54_RS11275 2414174..2414491 + 318 WP_009897465.1 hypothetical protein -
CWR54_RS11280 2414627..2415091 + 465 WP_011861514.1 hypothetical protein -
CWR54_RS11290 2415419..2415580 - 162 WP_004454578.1 hypothetical protein -
CWR54_RS11295 2415632..2416445 - 814 Protein_2131 toxin Bro -
CWR54_RS18900 2416565..2416717 - 153 WP_021361915.1 hypothetical protein -
CWR54_RS11300 2417990..2418913 - 924 WP_004454587.1 SHOCT domain-containing protein -
- 2419057..2419186 + 130 NuclAT_1 - Antitoxin
CWR54_RS11305 2419107..2419259 - 153 WP_004454589.1 hypothetical protein Toxin
CWR54_RS11310 2419533..2419856 - 324 WP_009897482.1 hypothetical protein -
CWR54_RS11315 2419892..2420146 - 255 WP_004454592.1 hypothetical protein -
CWR54_RS11325 2420578..2421096 - 519 Protein_2137 transposase -
CWR54_RS11330 2421386..2421760 - 375 Protein_2138 BlaI/MecI/CopY family transcriptional regulator -
CWR54_RS11335 2421865..2422239 - 375 WP_004454597.1 BlaI/MecI/CopY family transcriptional regulator -
CWR54_RS11340 2423043..2423531 + 489 WP_004454599.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
CWR54_RS18855 2423920..2424090 + 171 WP_021394397.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2411049..2429337 18288


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-133)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.92 Da        Isoelectric Point: 10.6365

>T89618 WP_004454589.1 NZ_CP025047:c2419259-2419107 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Download         Length: 153 bp

>T89618 NZ_CP025047:c2419259-2419107 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin


Download         Length: 130 bp

>AT89618 NZ_CP025047:2419057-2419186 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A125V725

Antitoxin

Download structure file

References