Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) CD-RCd/-
Location 2418065..2418267 Replicon chromosome
Accession NZ_CP025046
Organism Clostridioides difficile strain W0022a

Toxin (Protein)


Gene name CD2299.1 Uniprot ID Q185H1
Locus tag CWR55_RS11515 Protein ID WP_004454589.1
Coordinates 2418115..2418267 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name SQ1641
Locus tag -
Coordinates 2418065..2418194 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWR55_RS11480 2413314..2413631 + 318 WP_009897465.1 hypothetical protein -
CWR55_RS11485 2413767..2414231 + 465 WP_004454576.1 hypothetical protein -
CWR55_RS11495 2414560..2414721 - 162 WP_004454578.1 hypothetical protein -
CWR55_RS19840 2414773..2415587 - 815 Protein_2173 toxin Bro -
CWR55_RS19845 2415707..2415859 - 153 WP_009897477.1 hypothetical protein -
CWR55_RS11510 2416998..2417921 - 924 WP_021364626.1 SHOCT domain-containing protein -
- 2418065..2418194 + 130 NuclAT_5 - Antitoxin
CWR55_RS11515 2418115..2418267 - 153 WP_004454589.1 hypothetical protein Toxin
CWR55_RS11520 2418541..2418864 - 324 WP_021364628.1 hypothetical protein -
CWR55_RS11525 2418900..2419154 - 255 WP_004454592.1 hypothetical protein -
CWR55_RS11535 2419586..2420104 - 519 Protein_2179 transposase -
CWR55_RS11540 2420394..2420768 - 375 Protein_2180 BlaI/MecI/CopY family transcriptional regulator -
CWR55_RS11545 2420873..2421250 - 378 WP_021364350.1 BlaI/MecI/CopY family transcriptional regulator -
CWR55_RS11550 2422055..2422549 + 495 WP_021364347.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
CWR55_RS19760 2422938..2423108 + 171 WP_021416406.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2411493..2428357 16864


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.92 Da        Isoelectric Point: 10.6365

>T89606 WP_004454589.1 NZ_CP025046:c2418267-2418115 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Download         Length: 153 bp

>T89606 NZ_CP025046:c2418267-2418115 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin


Download         Length: 130 bp

>AT89606 NZ_CP025046:2418065-2418194 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A125V725

Antitoxin

Download structure file

References