Detailed information of TA system TA074675

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3784904..3785106
• Replicon: chromosome
• Accession: NZ_CP020424
• Organism: Clostridioides difficile strain FDAARGOS_267

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: A6J95_RS17675
• Protein ID: WP_004454589.1
• Coordinates: 3784904..3785056 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	3784977..3785106 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6J95_RS20275	3780074..3780244	-	171	WP_021394397.1	hypothetical protein	-
A6J95_RS17640	3780633..3781121	-	489	WP_009897487.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
A6J95_RS17645	3781925..3782299	+	375	WP_004454597.1	BlaI/MecI/CopY family transcriptional regulator	-
A6J95_RS17650	3782404..3782777	+	374	Protein_3330	BlaI/MecI/CopY family transcriptional regulator	-
A6J95_RS17655	3783067..3783585	+	519	Protein_3331	transposase	-
A6J95_RS17665	3784017..3784271	+	255	WP_004454592.1	hypothetical protein	-
A6J95_RS17670	3784307..3784630	+	324	WP_009897482.1	hypothetical protein	-
A6J95_RS17675	3784904..3785056	+	153	WP_004454589.1	hypothetical protein	Toxin
-	3784977..3785106	-	130	NuclAT_3	-	Antitoxin
A6J95_RS17680	3785250..3786134	+	885	WP_009897481.1	hypothetical protein	-
A6J95_RS20415	3787117..3787269	+	153	WP_009897477.1	hypothetical protein	-
A6J95_RS20420	3787389..3788203	+	815	Protein_3337	toxin Bro	-
A6J95_RS17695	3788255..3788416	+	162	WP_004454578.1	hypothetical protein	-
A6J95_RS17705	3788744..3789208	-	465	WP_009897467.1	hypothetical protein	-
A6J95_RS17710	3789344..3789661	-	318	WP_009897465.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_009089 Clostridium difficile 630 Prophage TA010176 2658361..2675497 0.01	inside	Prophage	-	-	3774825..3791482	16657

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T74675 WP_004454589.1 NZ_CP020424:3784904-3785056 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T74675 NZ_CP020424:3784904-3785056 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT74675 NZ_CP020424:c3785106-3784977 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin