Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) CD-RCd/-
Location 2510725..2510927 Replicon chromosome
Accession NZ_CP012320
Organism Clostridioides difficile strain DSM 28196

Toxin (Protein)


Gene name CD2299.1 Uniprot ID Q185H1
Locus tag CDIF28196_RS12035 Protein ID WP_004454589.1
Coordinates 2510775..2510927 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name SQ1641
Locus tag -
Coordinates 2510725..2510854 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDIF28196_RS12010 2506159..2506623 + 465 WP_011861514.1 hypothetical protein -
CDIF28196_RS12020 2506951..2507112 - 162 WP_004454578.1 hypothetical protein -
CDIF28196_RS12025 2507164..2507977 - 814 Protein_2214 toxin Bro -
CDIF28196_RS19830 2508097..2508249 - 153 WP_021361915.1 hypothetical protein -
CDIF28196_RS12030 2509658..2510581 - 924 WP_011861517.1 SHOCT domain-containing protein -
- 2510725..2510854 + 130 NuclAT_0 - Antitoxin
CDIF28196_RS12035 2510775..2510927 - 153 WP_004454589.1 hypothetical protein Toxin
CDIF28196_RS12040 2511201..2511524 - 324 WP_009897482.1 hypothetical protein -
CDIF28196_RS12045 2511560..2511814 - 255 WP_004454592.1 hypothetical protein -
CDIF28196_RS12055 2512250..2512768 - 519 Protein_2220 transposase -
CDIF28196_RS12060 2513058..2513433 - 376 Protein_2221 BlaI/MecI/CopY family transcriptional regulator -
CDIF28196_RS12065 2513538..2513915 - 378 WP_011861518.1 BlaI/MecI/CopY family transcriptional regulator -
CDIF28196_RS12070 2514719..2515213 + 495 WP_011861519.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
CDIF28196_RS12075 2515602..2515772 + 171 WP_004454601.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2502581..2524109 21528


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(36-258)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.92 Da        Isoelectric Point: 10.6365

>T56274 WP_004454589.1 NZ_CP012320:c2510927-2510775 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Download         Length: 153 bp

>T56274 NZ_CP012320:c2510927-2510775 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin


Download         Length: 130 bp

>AT56274 NZ_CP012320:2510725-2510854 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A125V725

Antitoxin

Download structure file

References